Title:Naringenin-induced Oral Cancer Cell Apoptosis Via ROS-mediated Bid
and Bcl-xl Signaling Pathway
Volume: 24
Issue: 6
Author(s): YuYe Du, Jia Lai, Jingyao Su, Jiali Li, Chuqing Li, Bing Zhu and Yinghua Li*
Affiliation:
- Center Laboratory, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, No 318 Renminzhong Road, Yuexiu District, Guangzhou, 510120, China
Keywords:
Naringenin, oral cancer, ROS, Bid, Bcl-xl, signaling pathway, apoptosis.
Abstract:
Background: Oral cancer is a malignant tumor with a high impact and poor prognosis.
Naringenin, a flavonoid found in citrus fruits and its anti-inflammatory and antioxidant properties
offer potential therapeutic benefits. However, limited studies have been conducted on the impact
of naringenin on human tongue carcinoma CAL-27 cells. This study aims to elucidate the correlation
between naringenin and tongue cancer, thereby identifying a potential therapeutic candidate
for drug intervention against tongue cancer.
Methods: The effect of naringenin on the apoptosis of CAL-27 cells and its mechanism were
studied by cell counting kit-8, mitochondrial membrane potential assay with JC-1, Annexin V--
FITC apoptosis detection, cell cycle, and apoptosis analysis, Reactive Oxygen Species assay and
Western blot.
Results: The results showed that naringenin significantly induced apoptosis in CAL-27 cells in a
dose-dependent manner. Mechanistically, naringenin-induced apoptosis was mediated through the
upregulation of Bid and downregulation of Bcl-xl, which led to increased generation of ROS.
Conclusion: The findings suggested that naringenin may represent a promising candidate for the
treatment of oral cancer by inducing apoptotic cell death via modulation of the Bid and Bcl-xl signaling
pathways.
