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Research Article

HMGB1/RAGE信号调控Th17/IL-17及其在支气管上皮-间质转化中的作用

卷 24, 期 11, 2024

发表于: 27 October, 2023

页: [1401 - 1412] 页: 12

弟呕挨: 10.2174/0115665240249953231024060610

价格: $65

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摘要

背景：气道重塑是与HMGB1/RAGE信号或Th17免疫相关的严重类固醇耐受性哮喘的原因之一。 目的：本研究旨在探讨HMGB1/RAGE信号通路与Th17/IL-17信号通路在气道重塑上皮-间充质转化（EMT）中的关系。 方法：采集C57小鼠的CD4+ T淋巴细胞。流式细胞术分析CD4+ T细胞与Th17细胞的比值。ELISA法检测IL-17水平。采用RT-qPCR和免疫组化方法对埃卡迪林和α-SMA进行分析。western blot检测Ecadherin、α-SMA、p-Smad3的表达。 结果：HMGB1/RAGE信号在体外以剂量依赖性方式促进Th17细胞的分化和成熟。HMGB1/RAGE信号也促进支气管EMT的发生。Th17/IL-17与HMGB1协同促进支气管上皮细胞的EMT。RAGE的沉默降低了HMGB1的信号转导和支气管EMT的进展。 结论：HMGB1/RAGE信号通过促进Th17细胞的分化和IL-17的分泌，协同增强TGF-β1诱导的支气管EMT。

关键词: HMGB1， RAGE, Th17/IL-17， EMT，气道重塑，支气管哮喘。

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