Title:Pre-operative Neo-adjuvant Chemotherapy Related miRNAs as Key
Regulators and Therapeutic Targets in Colorectal Cancer
Volume: 17
Issue: 1
Author(s): Orcun Yalav, Ozge Sonmezler, Kivilcim Eren Erdogan, Ahmet Rencuzogullari, Figen Doran, Atil Bisgin*Ibrahim Boga*
Affiliation:
- Department of Medical Genetics, Faculty of Medicine, Cukurova University AGENTEM (Adana Genetic Diseases Diagnosis and Treatment Center) & Cukurova University, Adana, Turkey
- AGENTEM (Adana Genetic Diseases Diagnosis and Treatment Center), Cukurova University, Adana, Turkey
- Department of Medical Genetics, Faculty of Medicine, Cukurova University AGENTEM (Adana Genetic Diseases Diagnosis and Treatment Center) & Cukurova University, Adana, Turkey
Keywords:
microRNA, colorectal cancer, biomarker, expression profiling, neoadjuvant therapy, therapeutic targets.
Abstract:
Background: In colorectal cancer, the investigation of cancer pathogenesis and the determination
of the relevant gene and gene pathways is particularly important to provide a basis for
treatment-oriented studies. miRNAs which affect gene regulation in the molecular pathogenesis of
cancer, have an active role in carcinogenesis. In the literature, miRNA expression levels have been
associated with metastasis and prognosis in different cancers.
Objective: In our study, expression profiling of miRNAs involved in oncogenic and apoptotic
pathways in patients with locally advanced colorectal cancer receiving neoadjuvant therapy was
performed.
Methods: miRNAs were isolated from three different FFPE tissue samples taken at different times
of the same patient (tumor tissue taken at the time of diagnosis, normal tissue samples, and after
neoadjuvant therapy). The expression analysis of 84 miRNAs determined by PCR array (Fluidigm,
USA) and mediated meta-analysis was performed comparatively to each study and non-cancerous
control group. Evaluations were performed with ΔΔCT calculations.
Results: As a result of the miRNA PCR array study, in addition to differences were observed in
miRNA expression between control and study groups. The potential biomarkers which were hsamiR-
215-5p, hsa-miR-9-59, hsa-miR-193a-5p, hsa-miR-206, hsa-miR-1, hsa-miR-96-5p have been
detected for possible treatment resistance, prognosis and predispositions to cancers.
Conclusion: In patients with colorectal cancer, miRNA expression in the tumoral regions before
and after neoadjuvant therapy has represented a variable pattern. It has been shown that miRNA
studies can be used to predict the clinical course and response to treatment with differences in expression
levels. It has been concluded that specific miRNAs may be candidate biomarkers for colorectal
cancer.
