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Research Article

MiR-148b通过抑制SIRT6表达导致外伤性失血性休克大鼠肝损伤

卷 24, 期 11, 2024

发表于: 19 October, 2023

页: [1390 - 1400] 页: 11

弟呕挨: 10.2174/1566524023666230816112629

价格: $65

摘要

背景：本研究旨在探讨miR- 148b在创伤性失血性休克（THS）大鼠肝损伤中的作用，并探讨其可能的机制。 方法：采用酶联免疫吸附试验（ELISA）检测大鼠血清中谷丙转氨酶（ALT）和天冬氨酸转氨酶（AST）水平，苏木精-伊红（H&E）染色分析大鼠肝脏损伤情况。采用末端脱氧核苷酸转移酶介导的dutp -生物素缺口末端标记法（TUNEL）和流式细胞术分别检测大鼠肝细胞和正常大鼠肝细胞系（BRL3A）的凋亡情况。采用定量逆转录聚合酶链反应（qRT-PCR）和Western blot检测MiR-148b和SIRT6的表达。乳酸脱氢酶（LDH）含量和细胞活力分别采用商用试剂盒和细胞计数试剂盒-8 （CCK-8）测定。通过Starbase数据库预测miR-148b和SIRT6的结合位点，并通过双荧光素酶报告基因试验进行验证。 结果：MiR-148b在THS大鼠或缺血再灌注（I/R）处理细胞中的表达高于对照组。过表达miR-148b进一步促进I/R的作用，使肝组织或BRL3A细胞的ALT、AST、LDH水平升高，细胞凋亡增加，SITR6表达降低。此外，miR-148b与SIRT6呈负相关，上调SIRT6的表达可部分逆转miR-148b的作用。 结论：I/R诱导的肝细胞损伤是通过调节miR-148b /SIRT6轴实现的。

关键词: miR-148b, SIRT6，外伤性失血性休克，I/R，细胞凋亡，肝损伤，丙氨酸转氨酶。

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