Title:PIWIL1 Promotes Malignant Progression of Papillary Thyroid Carcinoma
by Inducing EVA1A Expression
Volume: 24
Issue: 2
Author(s): Lianyong Liu, Fengying Wu, Xiaoying Zhang*Xiangqi Li*
Affiliation:
- Department of Nuclear
Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, China
- Department of Endocrinology and Metabolism, Gongli Hospital, Naval Medical University, Shanghai 200135, China
Keywords:
Papillary thyroid carcinoma, PIWIL1, EVA1A, qPCR, flow cytometry, apoptosis.
Abstract:
Introduction: Papillary thyroid carcinoma (PTC) is the most common subtype of thyroid
cancer. Previous studies have reported on the ectopic expression of P-element-induced wimpy testis
ligand 1 (PIWIL1) in various human cancers, but its role in PTC progression has not been investigated.
Methods: In this study, we measured the expression levels of PIWIL1 and Eva-1 homolog A (EVA1A)
in PTC using qPCR and WB. We performed a viability assay to evaluate PTC cell proliferation and
used flow cytometry to investigate apoptosis. Moreover, we conducted a Transwell invasion assay to
quantify cell invasion and assessed PTC growth in vivo using xenograft tumor models.
Results: Our findings showed PIWIL1 to be highly expressed in PTC and promote cell proliferation,
cell cycle activity, and cell invasion, while suppressing apoptosis. Additionally, PIWIL1 accelerated
tumor growth in PTC xenografts by modulating the EVA1A expression.
Conclusion: Our study suggests that PIWIL1 contributes to the progression of PTC through EVA1A
signaling, indicating its potential role as a therapeutic target for PTC. These results provide valuable
insights into PIWIL1 function and may lead to more effective treatments for PTC.
