Title:Maternal High Fat Diet and its Expressions in the Heart and Liver in
the Mice Embryogenesis
Volume: 24
Issue: 7
Author(s): Sanjeev Nirala*, Xue-Rui Tan, Muhammad Shafiq, Rajesh Basnet and Apekshya Singh
Affiliation:
- Department of Cardiology, First Affiliated Hospital of the Shantou University Medical College, Shantou,
515041, China
Keywords:
Maternal high fat diet, heart and liver, mice, embryogenesis, coronary artery disease, (lipoprotein lipase), SREBP1c.
Abstract:
Background: The developmental biology for the nonalcoholic fatty liver
disease and coronary heart disease are known but elaborative ideas of triglycerides
phenomenon in the embryo-genesis of the liver and the heart are still not clear.
Objective: The aim of the study was to relate different triglycerides like LXRα, LPL, LDL
R, PPARG-, SREBP-1C expression in the high fat fed mice with the normal fed diet
mice in the process of developmental and embryo-genesis biology.
Methods: Tissue preparation was done by ripalysis. Different protein content was
obtained via western blot for the 6 samples namely a-17.5 days mice embryo heart; b-
0th day or the birthday mice infant heart; c-1 week mice infant heart; d-2 weeks mice
infant heart; e-3 weeks mice infant heart; f-Adult mice heart. Protein lysates from the
heart tissues of the mice was obtained via homegenization and centrifugation.
Hematoxylin and Eosin (H and E) was done to see the fat droplets in the liver tissues at
the different developmental stages.
Result: LXRα,SREBP-1C expression in 17.5 days mice embryo heart and 0th day or
the birthday mice infant heart is highly expressed in the high fat diet. LDL-R in the high
fat diet mice is increased in 2 weeks mice infant heart but in17.5 days mice embryo
heart and in 0th day or the birthday mice infant heart it is low expression but from 1week
mice infant heart to the adult mice heart the expression is in decreasing trend. Similarly
LPL is highly expressed in17.5 days mice embryo heart and 1 week mice infant heart
and thus low expression in decreasing order until adult mice heart.Thus, these results
collectively shows that maternal HF diet increases expression of proteins such as LPL,
LDLr in the embryo phase and thus getting normal expressions in the adult phase that
facilitate Triglycerides (TAG) hydrolysis across the liver and the heart. Also,maternal
high fat diet increases the SREBP1c expression, leading to stimulation of LPL
Expression.
Conclusion: In summary, using a pregnant mice model, we found that maternal high fat
diet increases the fetal fat accumulation. Elevated placental LPL activity and expression
of genes that facilitate placental lipid transport suggest that enhanced placental lipid
transport may play a key role in maternal nutrition and obesity-induced fetal fat
accumulation.
