Title:Identification of miRNA-mRNA Regulatory Networks Associated with
Diabetic Retinopathy using Bioinformatics Analysis
Volume: 23
Issue: 13
Author(s): Weihai Xu, Ya Liang, Ying Zhuang and Zhilan Yuan*
Affiliation:
- Department
of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 224500, China
Keywords:
Diabetic retinopathy, microRNAs, ATP synthase, let-7a-5p, pathogenesis, diabetes mellitus.
Abstract:
Introduction: Diabetic retinopathy (DR) is a major complication of diabetes and a leading
cause of visual loss. This study aimed to explore biomarkers for DR that may provide additional
reference to DR pathogenesis and development.
Methods: The differentially expressed genes (DEGs) between the DR and control samples in the
GSE53257 dataset were identified. Logistics analyses were performed to identify DR-associated
miRNAs and genes, and correlation analysis was performed to determine the correlation between
them in GSE160306.
Results: A total of 114 DEGs in DR were identified in GSE53257. Three genes, including ATP5A1
(down), DAUFV2 (down), and OXA1L (down), were differentially expressed between DR and control
samples in GSE160306. Univariate logistics analysis identified that ATP5A1 (OR=0.007, p =
1.40E-02), NDUFV2 (OR = 0.003, p = 6.40E-03), and OXA1L (OR = 0.093, p = 3.08E-02) were
DR-associated genes. ATP5A1 and OXA1L were regulated by multiple miRNAs, of which hsa-let-
7b-5p (OR = 26.071, p = 4.40E-03) and hsa-miR-31-5p (OR = 4.188, p = 5.09E-02) were related to
DR. ATP5A1 and OXA1L were closely correlated with each other in DR.
Conclusion: The hsa-miR-31-5p-ATP5A1 and hsa-let-7b-5p-OXA1L axes might play novel and
important roles in the pathogenesis and development of DR.
