Title:Novel Insights of ANGPTL-3 on Modulating Cholesterol Efflux
Capacity Induced by HDL Particle
Volume: 24
Issue: 6
关键词:
ANGPTL3，胆固醇外排能力，HDL，稳定性心绞痛，2型糖尿病，冠状动脉疾病。
摘要:
Background: Angiopoietin-like protein 3 (ANGPTL-3) modulates lipid
metabolism and the risk of coronary artery disease (CAD), especially stable angina (SA),
via suppressing lipoprotein lipase (LPL). However, whether there are other mechanisms
is not elucidated yet. The current research explored the modulatory roles of ANGPTL-3
on high-density lipoprotein (HDL), which further affects atherosclerotic development.
Methods: A total of 200 individuals were enrolled in the present study. Serum ANGPTL-
3 levels were detected via enzyme-linked immunosorbent assays (ELISA). Cholesterol
efflux capacity induced by HDL particles was detected through H3-cholesterol loading
THP-1 cell.
Results: The serum ANGPTL-3 levels presented no significant discordance between the
SA group and the non-SA group, whereas the serum ANGPTL-3 levels in type 2
diabetes mellitus (T2DM) group were significantly elevated compared with those in the
non-T2DM group [428.3 (306.2 to 736.8) ng/ml vs. 298.2 (156.8 to 555.6) ng/ml, p
<0.05]. Additionally, the serum ANGPTL-3 levels were elevated in patients with low TG
levels compared to those in patients with high TG levels [519.9 (377.6 to 809.0) ng/ml
vs. 438.7 (329.2 to 681.0) ng/ml, p <0.05]. By comparison, the individuals in the SA
group and T2DM group presented decreased cholesterol efflux induced by HDL particles
[SA: (12.21±2.11)% vs. (15.51±2.76)%, p <0.05; T2DM: (11.24±2.13)% vs. (14.65±
3.27)%, p <0.05]. In addition, the serum concentrations of ANGPTL-3 were inversely
associated with the cholesterol efflux capacity of HDL particles (r=-0.184, p <0.05).
Through regression analysis, the serum concentrations of ANGPTL-3 were found to be
an independent modulator of the cholesterol efflux capacity of HDL particles
(standardized β=-0.172, p <0.05).
Conclusion: ANGPTL-3 exhibited a negative modulatory function on cholesterol efflux
capacity induced by HDL particles.
