Title:MicroRNA-34c-5p Reduces Malignant Properties of Lung Cancer
Cells through Regulation of TBL1XR1/Wnt/β-catenin Signaling
Volume: 24
Issue: 1
关键词:
癌症，微小RNA-34c-5p，增殖，迁移，侵袭，TBL1XR1。
摘要:
Introduction: Lung cancer is common cancer with high mortality. A growing
number of studies have focused on investigating the regulatory effects of microRNAs
(miRs/miRNAs) during cancer progression. Nevertheless, the biological function of miR-
34c-5p in lung cancer and the underlying mechanism have not been determined. This
study explored the effect of miR-34c-5p on the malignant behaviors of lung cancer cells.
Methods: In this study, we utilized diverse public databases to obtain differentially
expressed miRNAs. Then, qRT-PCR and western blot were conducted to determine
miR-34c-5p and transducin β-like 1 X-linked receptor 1 (TBL1XR1) expression. Next,
H1299 and H460 cells were transfected with miR-34c-5p-mimic and pcDNA3.1-
TBL1XR1. To examine the anticancer effects of miR-34c-5p, CCK-8, scratch, and
Matrigel-Transwell assays were conducted to test cell viability, migration, and invasion,
respectively. The StarBase database and dual-luciferase reporter gene assay were used
to predict and verify the relationship between miR-34c-5p and TBL1XR1.
Results: Finally, Wnt/β-catenin signaling- and epithelial-mesenchymal transition (EMT)-
related protein levels were detected using western blot. The results demonstrated that
miR-34c-5p was poorly expressed in lung cancer cells, while TBL1XR1 was highly
expressed. The findings also confirmed the direct interaction between miR-34c-5p and
TBL1XR1. In H1299 and H460 cells, miR-34c-5p overexpression inhibited cell
proliferation, migration, and invasion, Wnt/β-catenin signaling activity, and EMT, while
TBL1XR1 upregulation reversed these effects of miR-34c-5p overexpression.
Conclusion: These findings illustrated that miR-34c-5p might repress the malignant
behaviors of lung cancer cells via TBL1XR1, providing evidence for miR-34c-5p-based
lung cancer therapy.
