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Current Pharmaceutical Design

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ISSN (Print): 1381-6128
ISSN (Online): 1873-4286

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General Research Article

VK2 Promotes Osteogenic Differentiation of BMSCs against High Glucose Exposure via Modulation of Intracellular Oxidative Stress

Author(s): Wenlai Fang, Yiyun Lv, Shuqing Jin, Zheng Zhu and Yunzhen Chen*

Volume 29, Issue 9, 2023

Published on: 31 March, 2023

Page: [713 - 722] Pages: 10

DOI: 10.2174/1381612829666230328113007

Price: $65

Abstract

Introduction: Diabetic osteoporosis (DOP) has gradually gained public attention. The clinical manifestations of DOP include bone mass loss, bone microstructural damage, and increased bone fragility.

Methods: Intracellular reactive oxygen species (ROS) production was significantly increased under high glucose (HG) conditions, with deleterious effects on bone mesenchymal stem cells (BMSCs) proliferation and osteogenic differentiation. Vitamin K2 (VK2) has been demonstrated to promote bone formation both in vitro and in vivo.

Results: However, its potential role in diabetes-induced osteoporosis remains unelucidated. This study aims to verify whether VK2 treatment could relieve the deleterious effects of high glucose on BMSCs and delay the progression of osteoporosis. The results revealed that the HG environment downregulated the expression of osteogenesis- related proteins.

Conclusion: Correspondingly, VK2 treatment reversed the osteogenic phenotype of BMSCs under HG conditions. In addition, using an established diabetes-induced osteoporosis rat model, we found that VK2 administration could restore bone mass and microstructure. In conclusion, our results provide a promising therapeutic option in the clinical treatment of DOP.

Keywords: Diabetic osteoporosis (DOP), high glucose (HG), vitamin K2 (VK2), reactive oxygen species (ROS), mitochondrial dysfunction, bone mesenchymal stem cells (BMSCs).

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