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Research Article

脂多糖对不同年龄大鼠结肠的影响

卷 30, 期 39, 2023

发表于: 10 February, 2023

页: [4492 - 4503] 页: 12

弟呕挨: 10.2174/0929867330666230113112803

价格: $65

摘要

背景：脂多糖(lipopolaccharide, LPS)是一种引起炎症的内毒素，随着年龄的增长，脂多糖的含量逐渐增加。结肠对LPS刺激的反应是否会随着年龄的增长而增加尚不清楚。 目的：研究了LPS刺激对成年大鼠和老龄大鼠结肠的影响。 方法：选取健康雄性SD大鼠43只，4月龄时分为成年组和lps刺激成年组，22月龄时分为衰老组和lps刺激衰老组。腹腔注射LPS (1mg/kg)刺激大鼠24 h，观察结肠形态变化，检测肠道炎症反应、紧密连接蛋白、肠上皮细胞凋亡和增殖。 结果：LPS刺激后，成年大鼠结肠出现一系列形态学改变，炎症反应(TLR4、NF-κB、IL-1β)升高，紧密连接蛋白(ZO-1、Claudin1、Claudin2)水平改变，肠上皮细胞Bax、Bcl2和增殖(PCNA)增加。上述变化在衰老大鼠中也有发现。LPS刺激在一定程度上进一步促进衰老大鼠结肠的上述变化。 结论：在LPS刺激和衰老过程中，大鼠的一系列结肠变化被明显破坏，在LPS刺激的衰老大鼠中，这些变化在一定程度上进一步加重。

关键词: 脂多糖，衰老，炎症，紧密连接，凋亡，肠上皮细胞。脂多糖，衰老，炎症，紧密连接，凋亡，肠上皮细胞。

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