Title:Protective Effect of Chrysin Against Chlorpyrifos-induced Hepatotoxicity
in Rats: Biochemical and Histopathological Approaches in a Sub-chronic
Study
Volume: 24
Issue: 10
Author(s): Babak Roshanravan, Tahereh Farkhondeh, Majid Darroudi and Saeed Samarghandian*
Affiliation:
- Healthy Ageing Research Centre,
Neyshabur University of Medical Sciences, Neyshabur, Iran
Keywords:
Chrysin, chlorpyrifos, sub-chronic, hepatotoxicity, liver, histopathological.
Abstract:
Background: Chrysin (CH) is one of the important natural flavonoids with antioxidant
and anti-inflammatory activity. The aim of this study was to assess the protective effects of CH on
biochemical indexes and histopathological changes in the liver of male Wistar rats exposed to chlorpyrifos
(CPF).
Methods: We induced sub-chronic toxicity in rats using CPF (10 mg/kg/day, orally) and administrated
CH at 12.5, 25, and 50 mg/kg/day for 45 days.
Results: In this study, CPF increased liver enzyme activities compared with the control group (p <
0.05), and co-treated CH with CPF reduced them compared with the non-treated CPF group (p <
0.05). A significant reduction in the liver GSH concentration along with a significant elevation in the
concentrations of MDA and NO in the CPF group was observed compared with the control group (p
< 0.001). However, CH at a dose of 50 mg could reverse them nearly to the control group (p <
0.001). In the CPF, CPF + CH1, and CPF + CH2 groups, a marked (p < 0.05) increase was found in
the serum concentration of IL-6 compared with the control animals. No significant changes were
found in the IL-6 concentration of the CPF + CH3 compared with the controls. Moreover, the coadministration
of CH plus CPF induced histopathological alterations in liver.
Conclusion: These results suggest that CH attenuates hepatic enzymes and histopathological alterations
induced by CPF via modulating oxidative stress and inflammatory indices in rats.
