Title:Simultaneous Determination of Six Bioactive Components of Astragali
Radix in Rat Plasma Using Triple Quadrupole LC/MS and its Application
to a Pharmacokinetic Study
Volume: 18
Issue: 10
Author(s): Yanlan Chai*, Juan Wang, Jing Wang, Kaishuo Zhang and Zi Liu*
Affiliation:
- Department of Radiotherapy Oncology, Tianjin Medical University Cancer Institute and Cancer Hospital, National
Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research
Center for Cancer, Tianjin, 300060, P.R. China
- Department of Radiotherapy Oncology, The First Affiliated Hospital
of Xi'an Jiao Tong University, Xi'an, Shaanxi 710061, P.R. China;
Keywords:
Astragali Radix, triple quadrupole LC/MS, rat plasma pharmacokinetic, multiple reaction monitoring, acute radiation, Agilent Jet Steam System.
Abstract:
Background: To explore the pharmacokinetic data of Astragali Radix, a simple, one-step
deproteinization procedure was followed to prepare plasma samples, and separation was achieved on an
InfinityLab Poroshell 120 column (3.0 mm × 50 mm, 1.8 μm) with a gradient mobile phase consisting of
solution A (water containing 0.1% formic acid) and solution B (methanol) at a flow rate of 0.3 mL/min.
Aims: In this study, a rapid, sensitive and selective Triple Quadrupole LC/MS method was developed
to determine Calycosin-7-glucoside, Formononetin, Calycosin, Ononin, Cycloastragenoland
Astragaloside IV from the extractive of Astragali Radixin in rat plasma, and it was validated for rat
plasma as the matrix and applied for a pharmacokinetic study in rat plasma, while the internal standard
was Sulfamethoxazole.
Objective: Multiple reaction monitoring (MRM) was used with an electrospray ionization source
with the Agilent Jet Steam System (AJS-ESI) in the positive mode.
Methods: A rapid, sensitive and selective Triple Quadrupole LC/MS method was developed to determine
Calycosin-7-glucoside, Formononetin, Calycosin, Ononin, Cycloastragenoland Astragaloside IV from the
extractive of Astragali Radixin rat plasma, and it was validated for plasma as the matrix and applied for a
pharmacokinetic study in rats, while the internal standard was Sulfamethoxazole.
Results: There were different pharmacokinetic characteristics after orally administering AR, including
absorption and elimination rate, exposure degree, plasma distribution and retention rate.
Conclusion: Apart from that, a good linear response was observed within all analytes. The pharmacokinetic
study on the six analytes in rats after oral administration of Astragali Radix was successfully completed
by adopting this method, thus filling a blank in the pharmacokinetic studies of Astragali Radix.
