Title:BZW2, CDT1 and IVD Act As Biomarkers for Predicting Hepatocellular
Carcinoma
Volume: 23
Issue: 3
Author(s): Zhelong Jiang, Zhihong Wei, Jianwei Chen, Fang Yang, Yi Jiang and Lizhi Lv*
Affiliation:
- Department of Hepatobiliary Surgery, Fuzong Clinical College of Fujian Medical University, Fuzhou City, Fujian
Province, China
- Department of Hepatobiliary Surgery, 900 Hospital of the Joint Logistics Team, Fuzhou City, Fujian
Province, China
Keywords:
Biomarkers, hepatocellular carcinoma, BZW2, CDT1, IVD, liver transplantation.
Abstract:
Background: Hepatocellular carcinoma (HCC) is the leading cause of cancer-related deaths
globally. This study aimed to provide a comprehensive investigation to screen and identify biomarkers
for predicting HCC.
Methods: Firstly, the bioinformatics technique was applied to screen potential HCC-related genes, and
the relationships between BZW2, CDT1, IVD expression and survival rate and clinicopathological factors
were assessed. Afterward, qRT-PCR, western blot and immunohistochemistry were employed to
validate the expression of BZW2, CDT1, and IVD in clinical resected cancer specimens. Furthermore,
in vitro assays, cell cycle, apoptosis, colony formation and scratch experiments were performed to detect
the effects of si-BZW2, si-CDT1 and oe-IVD in HCC cells. In vivo experiments, tumor volume
and weight were measured to assess the anti-tumor effect of si-BZW2, si-CDT1 and oe-IVD in HCCtumor-
bearing mice.
Results: Bioinformatics analysis indicated that HCC patients with high expression of BZW2, CDT1
and low expression of IVD have a poor prognosis and unfavorable clinicopathological factors. Similarly,
clinical sample analysis revealed that BZW2 and CDT1 expression were increased while IVD expression
was decreased in HCC tissues. Meanwhile, in vitro experiments found that si-BZW2, si-
CDT1 and oe-IVD promoted apoptosis and inhibited the colony formation and migration of HCC cells.
As expected, in vivo experiments demonstrated that si-BZW2, si-CDT1 and oe-IVD could inhibit tumor
growth.
Conclusion: Increased BZW2, CDT1 levels, and decreased IVD levels could act as biomarkers for
predicting HCC. Furthermore, targeting BZW2, CDT1, and IVD may offer a new approach to treat
HCC.
