Title:P2RY12 Increased Neuroinflammation to Accelerate Depression-like
Behaviors by the NLPR3 Inflammasome
Volume: 19
Issue: 3
Author(s): Jie Liu and Enyan Yu*
Affiliation:
- Department
of Clinical Psychology, Zhejiang Cancer Hospital, Hanzhou 310012, China
Keywords:
P2RY12, NLPR3, depression-like behaviors, ubiquitination, proinflammatory cytokine, neurotrophils.
Abstract:
Introduction: Depression is a class of important mental illness, which has become a severe
health problem perplexing the world due to its high morbidity rate, high disability rate, and
great disease burden. This study aimed to evaluate the role and possible mechanisms of P2RY12 in
the depression-like behaviors model.
Methods: Serum samples of patients with depression-like behaviors were used to analyze the expression
of P2RY12. Models of mice were given LPS via intraperitoneal injection for 7 days. Behavioral
tests were executed in this experiment.
Results: The expression of P2RY12 in models of depression-like behaviors or mice with depression-
like behaviors were induced. The inhibition of P2RY12 presents depression-like behaviors and
reduces inflammation in the model of depression-like behaviors. P2RY12 induced NLRP3 expression
and suppressed NLRP3 ubiquitination in a model of depression-like behavior. The inhibition of
NLRP3 reduced the effects of P2RY12 in mice model of depression-like behaviors. The regulation
of NLRP3 controlled the effects of the P2RY12 in vitro model of depression-like behaviors.
Conclusion: We conclude that P2RY12 increased neuroinflammation to accelerate depression-like
behaviors by NLPR3 inflammasome, providing novel information for the treatment of depressionlike
behaviors.
