Login Register Cart 0
Generic placeholder image

Current Stem Cell Research & Therapy

Editor-in-Chief

ISSN (Print): 1574-888X
ISSN (Online): 2212-3946

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Platelet-rich Fibrin Promotes the Proliferation and Osteo-/odontoblastic Differentiation of Human Dental Pulp Stem Cells

Author(s): Jian Zhang*, Jiankun Wu, Xiuya Lin and Xueli Liu

Volume 18, Issue 4, 2023

Published on: 16 September, 2022

Page: [560 - 567] Pages: 8

DOI: 10.2174/1574888X17666220704092411

Price: $65

Open Access Journals Promotions 2
Abstract

Background: Pulp regeneration is a promising strategy that promotes the continued development of young permanent teeth with immature apical foramen. Platelet-rich fibrin (PRF) was found to stimulate the proliferation and differentiation of osteoblasts, but its effects on osteoblast/odontoblast differentiation of human dental pulp stem cells (hDPSCs) are unknown.

Methods: The hDPSCs were isolated and identified using known surface markers by flow cytometry. The CCK-8 assay and the expression of Ki67 and PCNA were used to examine hDPSC proliferation. After 7 days of culture in an osteo-/odontoblastic induction medium with various concentrations of liquid PRF (0, 10% and 20%), the early stage of osteogenesis-intracellular alkaline phosphatase (ALP) was checked. After 21 days of culture, matrix mineralization was checked using Alizarin Red S and quantified. The mRNA and protein levels of osteo-/odontoblastic genes, including RUNX2, DSPP, DMP1 and BSP, were measured by qRT-PCR. The notch signal was checked by Western blot to analyze three key proteins (Notch 1, Jagged 1 and Hes 1).

Results: PRF-treated groups showed higher expression of Ki-67 and PCNA, higher ALP activity, and the higher dose showed a stronger induction. PRF promoted osteo-/odontoblastic differentiation of hDPSCs indicated by elevated protein levels and mRNA levels of the expression of osteo-/odontoblastic markers. The three key proteins in Notch signaling showed an increase compared with the control group and increased as the PRF concentration increased.

Conclusion: PRF can promote the proliferation and osteo-/odontoblastic differentiation of hDPSC, which may be through the Notch signaling pathway.

Keywords: Platelet-rich fibrin, immature apical foramen, apical periodontitis, pulp regeneration, notch signaling, osteogenesis.

« Previous Next »
Graphical Abstract

[1]
Chandran V, Chacko V, Sivadas G. Management of a nonvital young permanent tooth by pulp revascularization. Int J Clin Pediatr Dent 2014; 7(3): 213-6.
[http://dx.doi.org/10.5005/jp-journals-10005-1268] [PMID: 25709305]
[2]
Chen MY, Chen KL, Chen CA, Tayebaty F, Rosenberg PA, Lin LM. Responses of immature permanent teeth with infected necrotic pulp tissue and apical periodontitis/abscess to revascularization procedures. Int Endod J 2012; 45(3): 294-305.
[http://dx.doi.org/10.1111/j.1365-2591.2011.01978.x] [PMID: 22077958]
[3]
Saoud TM, Martin G, Chen YH, et al. Treatment of mature permanent teeth with necrotic pulps and apical periodontitis using regenerative endodontic procedures: A case series. J Endod 2016; 42(1): 57-65.
[http://dx.doi.org/10.1016/j.joen.2015.09.015] [PMID: 26525552]
[4]
Kumar V, Zameer M, Prasad V, Mahantesh T. Boon of MTA Apexification in Young Permanent Posterior Teeth. Case Rep Dent 2014; 2014: 673127.
[http://dx.doi.org/10.1155/2014/673127] [PMID: 25431690]
[5]
Xie Z, Shen Z, Zhan P, et al. Functional dental pulp regeneration: Basic research and clinical translation. Int J Mol Sci 2021; 22(16): 8991.
[http://dx.doi.org/10.3390/ijms22168991] [PMID: 34445703]
[6]
Kang YH, Jeon SH, Park JY, et al. Platelet-rich fibrin is a Bioscaffold and reservoir of growth factors for tissue regeneration. Tissue Eng Part A 2011; 17(3-4): 349-59.
[http://dx.doi.org/10.1089/ten.tea.2010.0327] [PMID: 20799908]
[7]
Wu CL, Lee SS, Tsai CH, Lu KH, Zhao JH, Chang YC. Platelet-rich fibrin increases cell attachment, proliferation and collagen-related protein expression of human osteoblasts. Aust Dent J 2012; 57(2): 207-12.
[http://dx.doi.org/10.1111/j.1834-7819.2012.01686.x] [PMID: 22624763]
[8]
Naik B, Karunakar P, Jayadev M, Marshal VR. Role of Platelet rich fibrin in wound healing: A critical review. J Conserv Dent 2013; 16(4): 284-93.
[http://dx.doi.org/10.4103/0972-0707.114344] [PMID: 23956527]
[9]
Liu Y, Sun X, Yu J, et al. Platelet-rich fibrin as a bone graft material in oral and maxillofacial bone regeneration: classification and summary for better application. BioMed Res Int 2019; 2019: 3295756.
[http://dx.doi.org/10.1155/2019/3295756] [PMID: 31886202]
[10]
Banyatworakul P, Osathanon T, Chumprasert S, Pavasant P, Pirarat N. Responses of canine periodontal ligament cells to bubaline blood derived platelet rich fibrin in vitro. Sci Rep 2021; 11(1): 11409.
[http://dx.doi.org/10.1038/s41598-021-90906-z] [PMID: 34075114]
[11]
Thanasrisuebwong P, Kiattavorncharoen S, Surarit R, Phruksaniyom C, Ruangsawasdi N. Red and yellow injectable platelet-rich fibrin demonstrated differential effects on periodontal ligament stem cell proliferation, migration, and osteogenic differentiation. Int J Mol Sci 2020; 21(14): E5153.
[http://dx.doi.org/10.3390/ijms21145153] [PMID: 32708242]
[12]
Kobayashi E, Flückiger L, Fujioka-Kobayashi M, et al. Comparative release of growth factors from PRP, PRF, and advanced-PRF. Clin Oral Investig 2016; 20(9): 2353-60.
[http://dx.doi.org/10.1007/s00784-016-1719-1] [PMID: 26809431]
[13]
Li C, Xie X, Liu Z, Yang J, Zuo D, Xu S. Neu5Ac induces human dental pulp stem cell osteo-/odontoblastic differentiation by enhancing MAPK/ERK pathway activation. Stem Cells Int 2021; 2021: 5560872.
[http://dx.doi.org/10.1155/2021/5560872] [PMID: 34603453]
[14]
Niapour A, Ghasemi Hamidabadi H, Niapour N, Mohammadi P, Sharifi Pasandi M, Malekzadeh V. Pharmacological notch pathway inhibition leads to cell cycle arrest and stimulates ascl1 and neurogenin2 genes expression in dental pulp stem cells-derived neurospheres. Biotechnol Lett 2019; 41(6-7): 873-87.
[http://dx.doi.org/10.1007/s10529-019-02687-1] [PMID: 31073804]
[15]
Hang K, Ye C, Xu J, et al. Apelin enhances the osteogenic differentiation of human bone marrow mesenchymal stem cells partly through Wnt/β-catenin signaling pathway. Stem Cell Res Ther 2019; 10(1): 189.
[http://dx.doi.org/10.1186/s13287-019-1286-x] [PMID: 31238979]
[16]
Liu J, Sato C, Cerletti M, Wagers A. Notch signaling in the regulation of stem cell self-renewal and differentiation. Curr Top Dev Biol 2010; 92: 367-409.
[http://dx.doi.org/10.1016/S0070-2153(10)92012-7] [PMID: 20816402]
[17]
Zhou S, Chen S, Jiang Q, Pei M. Determinants of stem cell lineage differentiation toward chondrogenesis versus adipogenesis. Cell Mol Life Sci 2019; 76(9): 1653-80.
[http://dx.doi.org/10.1007/s00018-019-03017-4] [PMID: 30689010]
[18]
VanDussen KL, Carulli AJ, Keeley TM, et al. Notch signaling modulates proliferation and differentiation of intestinal crypt base columnar stem cells. Development 2012; 139(3): 488-97.
[http://dx.doi.org/10.1242/dev.070763] [PMID: 22190634]
[19]
Tian Y, Xu Y, Xue T, et al. Notch activation enhances mesenchymal stem cell sheet osteogenic potential by inhibition of cellular senescence. Cell Death Dis 2017; 8(2): e2595.
[http://dx.doi.org/10.1038/cddis.2017.2] [PMID: 28151468]
[20]
Wang X, Jiao Y, Pan Y, et al. Fetal dermal mesenchymal stem cell-derived exosomes accelerate cutaneous wound healing by activating notch signaling. Stem Cells Int 2019; 2019: 2402916.
[http://dx.doi.org/10.1155/2019/2402916] [PMID: 31281370]
[21]
Wang J, Wang CD, Zhang N, et al. Mechanical stimulation orchestrates the osteogenic differentiation of human bone marrow stromal cells by regulating HDAC1. Cell Death Dis 2016; 7: e2221.
[http://dx.doi.org/10.1038/cddis.2016.112] [PMID: 27171263]
[22]
Ziouti F, Ebert R, Rummler M, et al. NOTCH signaling is activated through mechanical strain in human bone marrow-derived mesenchymal stromal cells. Stem Cells Int 2019; 2019: 5150634.
[http://dx.doi.org/10.1155/2019/5150634] [PMID: 30936923]
[23]
Miron RJ, Fujioka-Kobayashi M, Bishara M, Zhang Y, Hernandez M, Choukroun J. Platelet-rich fibrin and soft tissue wound healing: a systematic review. Tissue Eng Part B Rev 2017; 23(1): 83-99.
[http://dx.doi.org/10.1089/ten.teb.2016.0233] [PMID: 27672729]
[24]
Shah R. M G T, Thomas R, Mehta DS. An update on the protocols and biologic actions of platelet rich fibrin in dentistry. Eur J Prosthodont Restor Dent 2017; 25(2): 64-72.
[http://dx.doi.org/10.1922/EJPRD_01690Shah09] [PMID: 28590091]
[25]
Kargarpour Z, Nasirzade J, Di Summa F, Panahipour L, Miron RJ, Gruber R. Platelet-rich fibrin can neutralize hydrogen peroxide-induced cell death in gingival fibroblasts. Antioxidants 2020; 9(6): E560.
[http://dx.doi.org/10.3390/antiox9060560] [PMID: 32604944]
[26]
Strauss FJ, Stähli A, Gruber R. The use of platelet-rich fibrin to enhance the outcomes of implant therapy: A systematic review. Clin Oral Implants Res 2018; 29 (Suppl. 18): 6-19.
[http://dx.doi.org/10.1111/clr.13275] [PMID: 30306698]
[27]
Verma UP, Yadav RK, Dixit M, Gupta A. Platelet-rich fibrin: A paradigm in periodontal therapy - a systematic review. J Int Soc Prev Community Dent 2017; 7(5): 227-33.
[http://dx.doi.org/10.4103/jispcd.JISPCD_429_16] [PMID: 29026693]
[28]
Couto de Carvalho LA, Tosta Dos Santos SL, Sacramento LV, et al. Mesenchymal stem cell markers in periodontal tissues and periapical lesions. Acta Histochem 2020; 122(8): 151636.
[http://dx.doi.org/10.1016/j.acthis.2020.151636] [PMID: 33132168]

Rights & Permissions Print Cite
Article Metrics
75
2
Wayfinder Image
Open Access Journals Promotions
© 2024 Bentham Science Publishers | Privacy Policy