Title:Protective Effect of Artemisia absinthium Extract Against Glutamate-
Induced Toxicity in PC12 Cells
Volume: 20
Issue: 8
Author(s): Roghayeh Rashidi, Maryam Akaberi, Leila Mobasheri, Mahmood Sadeghi, Seyed Hadi Mousavi and Fatemeh Forouzanfar*
Affiliation:
- Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
- Department of Neuroscience,
Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Keywords:
Artemisia absinthium, PC12 cells, glutamate, antioxidant, apoptosis, central nervous system disorders.
Abstract:
Objectives: Glutamatergic dysfunction has been implicated in the pathogenesis of numerous
central nervous system disorders. Artemisia absinthium (A. absinthium), has been reported to have neuroprotective
and antioxidant activities. The current study examined the effect of A. absinthium extract on
glutamate-induced cytotoxicity using PC12 cells.
Methods: PC12 cells were pretreated with A. absinthium extract (70% ethanol) at concentration ranges of
6.25-25 μg/ml for 2 h, followed by exposure to glutamate (8 mM) for 24 hr. The cells viability, reactive
oxygen species (ROS) accumulation, and lipid peroxidation were measured. Moreover, a propidium iodide
flow cytometry assay was performed for the assessment of apoptosis.
Results: Pretreatment with A. absinthium extract markedly attenuated the loss of cell viability, ROS generation,
lipid peroxidation, and cell apoptosis in glutamate-injured PC-12 cells.
Conclusion: Our results indicate that A. absinthium extract protects PC12 cells against glutamate-induced
injury, possibly by reducing oxidative stress and apoptosis.
