Title:The Anticonvulsant Activity of Thiamine, Vitamin D3, and Melatonin
Combination on Pentylenetetrazole-induced Seizures in Mice
Volume: 17
Issue: 4
Author(s): Saeed Mehrzadi, Ehsan Dehdashtian, Mohammad Yahya Karimi and Azam Hosseinzadeh*
Affiliation:
- Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran
Keywords:
Seizures, pharmacology, melatonin, thiamine, vitamin D3, oxidative stress.
Abstract:
Objective: Epilepsy is a neurological disorder associated with the elevation of oxidative
stress levels and alteration in vitamins and endogenous antioxidant levels. The current study was designed
to evaluate the effects of Vit D3, thiamine, melatonin, and their combination on pentylenetetrazol
(PTZ)-induced tonic-clonic seizures in mice along with measuring the alteration in oxidative
stress markers.
Methods: Male mice were randomly divided into seven groups; group I received normal saline (0.5
ml, i.p.) on the 15th day, group II received PTZ (60 mg/kg, i.p) on the 15th day, groups III-VI received
diazepam (4 mg/kg), Vit D3 (6000 IU/kg, p.o.), thiamine (200 mg/kg, p.o.), and melatonin (20 mg/kg,
p.o.), respectively, before PTZ (60 mg/kg, i.p.) injection on the 15th day, and group VII received the
combination of Vit D3, thiamine, and melatonin, before PTZ injection on the 15th day. After PTZ injection,
the seizure latency and duration were measured. The malondialdehyde (MDA) level and superoxide
dismutase (SOD) activity were evaluated in mice brains.
Results: In the treatment group, the seizure latency was greater and the seizure duration was lower
than in the PTZ group. These agents decreased the MDA level and increased SOD activity compared
to the PTZ group. The combination of these agents was more effective than each alone to increase seizure
latency and reduce seizure duration.
Conclusion: The combination of Vit D3, thiamine, and melatonin could improve PTZ-induced seizures
more effectively than each one alone; this may be mediated by inhibiting oxidative stress and
regulating various mechanisms involved in seizures.
