Title:The Role of Human Platelet-rich Plasma to Enhance the Differentiation of
Adipose-derived Mesenchymal Stem Cells into Cardiomyocyte: An Experimental
Study
Volume: 21
Issue: 3
Author(s): I.Gde Rurus Suryawan, Andrianto*, Arifta Devi Anggaraeni, Arisya Agita and Ricardo Adrian Nugraha
Affiliation:
- Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General
Hospital, Surabaya, Indonesia
Keywords:
Adipocyte-derived mesenchymal stem cells, cardiac troponin-T, GATA-4, platelet-rich plasma, growth factor, cardiac regeneration.
Abstract:
Background: Several studies have shown that adipose-derived mesenchymal stem cells
(AMSCs) can differentiate into mesenchymal lineages, including cardiac cell types, but complete
differentiation into cardiomyocytes is challenging. Unfortunately, the optimal method to maximize
AMSCs differentiation has not yet been established. Platelet-rich plasma (PRP), which contains
rich growth factors, is believed to stimulate stem cell proliferation and differentiation in the context
of cardiac tissue regeneration.
Objective: This study aimed to analyze the effect of PRP administration to enhance the differentiation
of AMSCs into cardiomyocytes.
Methods: This study used a randomized post-test-only controlled group design. AMSCs were isolated
from adipose tissues and cultured for 4 passages. The samples were divided into 3 groups, a
negative control group (α-MEM), a positive control group (differentiation medium), and a treatment
group (PRP). The assessment of GATA-4 expression was conducted using flow cytometry on
day-5. The assessment of troponin expression was conducted using immunocytochemistry on day-
10. Data analysis was conducted using T-test and One-Way ANOVA.
Results: Flowcytometry of GATA-4 expression revealed a significant improvement in PRP group
compared to negative and positive control group (67.04 ± 4.49 vs. 58.15 ± 1.23 p < 0.05; 67.04 ±
4.49 vs. 52.96 ± 2.02 p < 0.05). This was supported by the results of immunocytochemistry on
troponin expression, which revealed significant improvement in the PRP group compared to negative
and positive controls (38.13 ± 5.2 vs. 10.73 ± 2.39 p < 0.05; 38.13 ± 5.2 vs. 26.00 ± 0.4 p <
0.05).
Conclusion: PRP administration in the AMSCs culture could significantly improve the differentiation
of AMSCs into cardiomyocytes measured by GATA-4 and troponin expressions. This was
concordant with our hypothesis, which stated that there was an effect of PRP administration on
AMSCs differentiation into cardiomyocytes.
