Title:Degree of Gelatination on Ag-Nanoparticles to Inactivate Multi-drug
Resistant Bacterial Biofilm Isolated from Sewage Treatment Plant
Volume: 20
Issue: 5
Author(s): Nagaraj Saraswathi, Koyeli Girigoswami, Kurunchi Chellapathi Divya, Subbaraj Gowtham Kumar and Agnishwar Girigoswami*
Affiliation:
- Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute (CHRI), Chettinad Academy of Research
and Education (CARE), Kelambakkam, Chennai 603 103, India
Keywords:
Multidrug resistant, gelatin, silver nanoparticles, Pseudomonas aeruginosa, biofilm, sewage treatment.
Abstract:
Introduction: Overuse and improper dosage of antibiotics have generated antimicrobial
resistance (AMR) worldwide. Pseudomonas aeruginosa (PA), a well-known bacterial strain can establish
MDR leading to a variety of infections in humans. Furthermore, these PA strains hold the ability to
form biofilms by generating extracellular polymeric substances on the surface of medical tools and
critical care units. To supersede the infectious effect of MDR organisms, silver nanoparticles have been
known to be the choice.
Materials and Methods: Hence, the present study concentrates on the engineering of varying concentrations
of gelatin-based polymeric hydrogel embedded with silver nanoparticles (G-AgNPs) for controlled
bactericidal activity against MDR PA biofilms. Biofilms formation by MDR PA was confirmed
microscopically and spectroscopy was taken as a tool to characterize and analyze the efficacy at every
stage of experiments.
Results: When MDR PA biofilms were treated with G-AgNPs prepared with 5 % gelatin concentration
(AgNP3), they exhibited superior bactericidal activity. Furthermore, a dose-dependent study showed
that 800 nM of AgNP3 could inhibit the growth of MDR PA.
Conclusion: Hence it can be concluded that silver nanoparticles synthesized in the presence of 5%
gelatin can act as a bactericidal agent in the inactivation of MDR PA biofilms, thereby controlling the
infections caused by these biofilms.