Title:iTRAQ-Based Quantitative Proteomics Analysis Reveals the Invasion
Mechanism of Spiroplasma eriocheiris in 3T6 Cells
Volume: 19
Issue: 3
Author(s): Peng Liu, Youyuan Ye, Shasha Xiang, Yuxin Li, Chengbin Zhu, Zixu Chen, Jie Hu, Ye Gen, Li Lou, Xuqi Duan, Juan Zhang*Wei Gu*
Affiliation:
- Institute of Pathogenic Biology, Hengyang Medical College, Institute of Pharmacy and Pharmacology, University of
South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative
Innovation Center for Molecular Target New Drug Study, Hengyang 421001, Hunan, China
- Jiangsu Key Laboratory
for Microbes & Functional Genomics and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College
of Life Sciences, Nanjing Normal University, No.1 Wenyuan Road, 210046 Nanjing, China
- Co-Innovation Center for
Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, 222005 Jiangsu, China
Keywords:
3T6 cell, iTRAQ, Spiroplasma eriocheiris, pathogenic mechanism, GO term analysis, KEGG pathway analysis.
Abstract:
Background: Spiroplasma eriocheiris is a novel pathogen of freshwater crustaceans and
is closely related to S. mirum. They have no cell wall and a helical morphology. They have the ability
to infect mammals with an unclear mechanism.
Objective: In this study, our aim was to investigate the profile of protein expression in 3T6 cells infected
with S. eriocheiris.
Methods: The proteome of 3T6 cells infected by S. eriocheiris was systematically investigated by
iTRAQ.
Results: We identified and quantified 4915 proteins, 67 differentially proteins were found, including
30 up-regulated proteins and 37 down-regulated proteins. GO term analysis shows that dysregulation
of adhesion protein , interferon and cytoskeletal regulation are associated with apoptosis. Adhesion
protein Vcam1 and Interferon-induced protein GBP2, Ifit1, TAPBP, CD63 ,Arhgef2 were
up-regulated. A key cytoskeletal regulatory protein, ARHGEF17 was down-regulated. KEGG pathway
analysis showed the NF-kappa B signaling pathway, the MAPK signaling pathway , the Jak-STAT
signaling pathway and NOD-like receptor signaling are closely related to apoptosis in vivo.
Conclusion: Analysis of the signaling pathways involved in invasion may provide new insights for
understanding the infection mechanisms of S. eriocheiris.
