Title:In Vitro Effects of Propofol on Cytotoxic, Apoptotic and PI3K-Akt Signaling Pathway
Genes on Brain Cancer Cells
Volume: 22
Issue: 2
Author(s): Tuba Gokdogan Edgunlu, Cigir Biray Avci*, Neslihan Pınar Ozates, Bakiye Goker Bagca, Sevim Karakas Celik, Aydin Boluk and Bakiye Ugur
Affiliation:
- Department of Medical Biology,
Faculty of Medicine, Ege University, Bornava, Turkey
Keywords:
Propofol, PI3K-AKT, glioblastoma, gene expression, signaling pathway, apoptosis.
Abstract: Aim: The study aimed to determine the cytotoxic and apoptotic effect of propofol on glioma cells.
Background: Propofol [2,6-diisopropylphenol] is a commonly used intravenous anesthetic. Propofol is known to have
a mechanism of action on the PI3K-AKT pathway.
Objective: This study aimed to evaluate the effect of propofol on the proliferation and apoptosis of human glioma cells,
as well as to investigate changes in expression levels of the PI3K-AKT signaling pathway genes.
Materials and Methods: The cytotoxic effect of propofol on the U-87 MG cell line was determined by WST-1 method.
Annexin V-FITC and Mitoprobe JC-1 assay were used to measure apoptosis by flow cytometry. The expression levels
of genes in the PI3K-AKT signaling pathway were investigated by qRT-PCR.
Results: We have shown that propofol induced apoptosis in U-87 MG cells by 17.1 fold compared to the untreated
control. Furthermore, significant differences were found in the expression levels of the PI3K-AKT signaling pathway
genes.
Conclusion: As a result of our study, it was found that propofol caused differences in expression levels of PI3K-AKT
signaling pathway genes and it was suggested that these differences may be related to apoptosis induction.
