Title:Organoids as Ex Vivo Culture System to Investigate Infection-host Interaction in Gastric Pre-carcinogenesis
Volume: 15
Issue: 2
Author(s): Cristina Di Giorgio*, Rosalinda Roselli, Michele Biagioli, Silvia Marchianò, Eleonora Distrutti, Martina Bordoni, Annibale Donini and Stefano Fiorucci
Affiliation:
- Dipartimento di Medicina e Chirurgia, Università di Perugia, Perugia, Italy
Keywords:
Organoids, Helicobacter pylori, EBV, gastric cancer, infection, host-pathogen interaction.
Abstract: Advancements in stem cell research have enabled the establishment of three-dimensional
(3D) primary cell cultures, known as organoids. These culture systems follow the organization
of an in vivo organ, as they enclose the different epithelial cell lines of which it is normally composed.
The generation of these 3D cultures has bridged the gap between in vitro models, made up
of two-dimensional (2D) cancer cell lines cultures, and in vivo animal models, with major differences
regarding human diseases. Organoids are increasingly used as a model to study colonization
of gastric mucosa by infectious agents and better understand host-microbe interactions and the
molecular events that lead to infection, pathogen-epithelial cells interactions, and mechanisms of
gastric mucosal injury. In this review, we will focus on the role of organoids as a tool to investigate
molecular interactions of Helicobacter (H.) pylori and Epstein Barr Virus (EBV) and gastric mucosa
and how these infections, which affect ≈ 45 % of the world population, might progress to gastric
cancer, a highly prevalent cancer and the third leading cause of cancer death.
