Title:Advanced Platelet-Rich Fibrin Extract Treatment Promotes the Proliferation
and Differentiation of Human Adipose-Derived Mesenchymal Stem
Cells through Activation of Tryptophan Metabolism
Volume: 18
Issue: 1
Author(s): Guan-Ming Lu, Li-Yuan Jiang, Dong-Lin Huang, Yong-Xian Rong, Yang-Hong Li, Liu-Xing Wei, Yan Ning, Shan-Fu Huang, Steven Mo*, Fu-Han Meng*Hong-Mian Li*
Affiliation:
- Yuan Dong International Academy of Life Sciences, Nanning,
China
- Department of Rehabilitation Medicine, The People’s Hospital of Binyang County, Binyang, Guangxi, 530405,
China
- Research Center of Medical Sciences, The People’s Hospital of Guangxi Zhuang Autonomous Region
& Guangxi Academy of Medical Sciences, Nanning 530021, China
Keywords:
Adipose-derived stem cell, advanced-platelet-rich fibrin extract, proliferation, differentiation, pathway, clinic.
Abstract:
Background: Advanced platelet-rich fibrin extract (APRFE) contains a high concentration
of various cytokines that are helpful for improving stem cells repair function.
Objective: However,
the underlying mechanism of APRFE improving stem cell repairing is not clear.
Methods: We produced APRFE by centrifuging fresh peripheral blood samples and isolated and
identified human adipose-derived mesenchymal stem cells (ADMSCs). The abundance of cytokines
contained in APRFE was detected by the Enzyme-linked immunosorbent assay (ELISA).
The ADMSCs treated with or without APRFE were collected for transcriptome sequencing.
Results: Based on the sequencing data, the expression profiles were contracted. The differentially
expressed genes and lncRNA (DEGs and DElncRNAs) were obtained using for the differential expression
analysis. The lncRNA-miRNA-mRNA network was constructed based on the miRNet
database. The further enrichment analysis results showed that the biological functions were mainly
related to proliferation, differentiation, and cell-cell function. To explore the role of APRFE, the
protein-protein interaction network was constructed among the cytokines included in APRFE and
DEGs. Furthermore, we constructed the global regulatory network based on the RNAInter and TRRUST
database. The pathways in the global regulatory network were considered as the core pathways.
We found that the DEGs in the core pathways were associated with stemness scores.
Conclusion: In summary, we predicted that APRFE activated three pathways (tryptophan
metabolism, mTOR signaling pathway, and adipocytokine signaling) to promote the proliferation
and differentiation of ADMSCs. The finding may be helpful for guiding the application of ADMSCs
in the clinic.
