Title:Difference of MreBCD Complex Interactome in Salmonella Typhimurium
ST19 and ST213 Genotypes on Pathogenesis and Stress Response Pathways
Volume: 22
Issue: 11
Author(s): Reyna C. Zepeda-Gurrola, Gerardo Vázquez-Marrufo, Xianwu Guo, Isabel C. Rodríguez-Luna, Alejandro Sánchez-Varela and Ma. Soledad Vázquez-Garcidueñas*
Affiliation:
- División de Estudios de Posgrado, Facultad de Ciencias Médicas y Biológicas “Dr. Ignacio Chávez”, Universidad
Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, C. P. 58020, Mexico
Keywords:
Flagella-mediated bacterial motility, prokaryotic cytoskeleton, protein prediction interaction, RcsC, stress response, two-hybrid assay.
Abstract:
Background: Salmonella enterica is the etiological agent of salmonellosis, with a high
infection rate worldwide in Mexico, ST213 genotype of S. enterica ser. Typhimurium is displacing
the ancestral ST19 genotype. Bacterial cytoskeleton protein complex MreBCD plays an important
role in S. enterica pathogenesis, but underlying mechanisms are unknown.
Results: In this study, 106 interactions among MreBCD and 15 proteins from S. Typhimurium
Pathogenicity Islands 1 (SP-I) and 2 (SP-2) involved in both bacterial virulence and stress response
were predicted in ST213 and ST19 genotypes, of which 12 interactions were confirmed in vitro. In
addition, gene cluster analysis in 100 S. Typhimurium genomes was performed for these genes.
Results and Conclusion: The in silico and in vitro results showed a novel MreBCD interactome involved
in regulating pathogenesis and stress response through interactions with virulence factors located
at SPI-1 and SPI-2. Furthermore, both pseudogene presence and sequence variations in four
tested proteins between genotypes resulted in differential interaction patterns involved in Salmonella
motility and survival in eukaryotic cells, which could explain the replacement of ST19 by
ST213 in Mexico.
