Airway Fibroblast Secretory Products Enhance Cell Migration

Nundisa       Jaulin; Ruszymah    Hj    Idrus; Aminuddin       Saim; Wan    Izlina    Wan-Ibrahim; Puteri    Shafinaz    Abdul-Rahman; Yogeswaran       Lokanathan

doi:10.2174/1570164618666210823094105

Abstract

Background: The nasal fibroblast secretome, which includes various cytokines, chemokines, and growth factors, promotes cell migration. Currently, the proteomics of Airway Fibroblast (AF) Conditioned Medium (AFCM) are being actively studied.

Objective: This study was aimed at profiling and identifying the AF secreted proteins that can enhance wound healing of the airway epithelium and predict the potential pathway involved.

Methods: Airway Epithelial Cells (AECs) and AFs were isolated from redundant human nasal turbinate and cultured. AFCM was collected by culturing the AFs either with serum-free airway epithelium basal medium (AECM) or with serum-free F12:DMEM (FDCM). For evaluating cell migration, the AECs were supplemented with airway epithelium medium and defined keratinocyte medium (1:1; AEDK; control), or with AEDK supplemented with 20% AECM or 20% FDCM. The mass spectrometry sample was prepared by protein precipitation, followed by gel electrophoresis and in-gel digestion.

Results: AECM promoted better cell migration compared to the FDCM and the control medium. Bioinformatics analysis identified a total of 121, and 92 proteins from AECM and FDCM, respectively: 109 and 82 were identified as secreted proteins, respectively. STRING® analysis predicted that 23 proteins from the AECM and 16 proteins from the FDCM are involved in wound healing.

Conclusion: Conditioned medium promotes wound healing by enhancing cell migration, and we successfully identified various secretory proteins in a conditioned medium that play important roles in wound healing.

Keywords: Secretome, conditioned medium, cell migration, wound healing, fibroblasts, proteomics, airway epithelium.

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DOI https://dx.doi.org/10.2174/1570164618666210823094105	Print ISSN 1570-1646
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Airway Fibroblast Secretory Products Enhance Cell Migration

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