Title:Evaluation of the Role of Ghrelin and Leptin as Biochemical Markers in
Female with Polycystic Ovarian Syndrome
Volume: 20
Issue: 4
Author(s): Mahmod Khalil Ibrahim and Amina Hamed Ahmed Alobaidi*
Affiliation:
- Department of Biochemistry and Pharmacology, Kirkuk University College of Veterinary Medicine, Kirkuk, Iraq
Keywords:
Adiponectin, follicular stimulating hormone, luteinizing hormone, prolactin, testosterone, leptin
Abstract: Introduction: Polycystic Ovary Syndrome (PCOS) is the most common endocrine disorder
in women of reproductive age. PCOS is a heterogeneous complex disorder of defined aetiology.
Some studies report its association with various endocrine, metabolic and immunological abnormalities.
The hunger hormones ghrelin and leptin affect the pathogenesis of PCOS and might lead
to the development of Metabolic Syndrome (MS) in obese women.
Aim: The study aims at evaluating the role of ghrelin and leptin level in females with polycystic
ovary syndrome as a biochemical marker for the diagnosis and monitoring progression.
Subjects and Methods: The study included one hundred PCOS patients and fifty apparently
healthy subjects with regular menstrual cycle, visiting gynecology outpatient clinic of Kalar General
Hospital, from the beginning of February 2015 to the end of June 2015. Body Mass Index (BMI)
along with serum ghrelin, leptin, Luteinizing Hormone (LH), Follicle Stimulating Hormone (FSH)
and testosterone levels were measured for both groups. Serum leptin was determined using sandwich
ELISA, while serum ghrelin was determined using competitive ELISA. Differences between
patient and control groups were assessed by using a t-test. Also, one-way Anova was used to test
the relationships among different groups.
Results: A significant decrease in ghrelin level and an increase in leptin levels in women with
PCOS were observed compared to the control group. Also, a significant elevation in serum levels
of LH, Testosterone, Prolactin, and a decrease in serum FSH in PCOS patients were observed,
when compared to the control group. Additionally, serum ghrelin decreased and serum leptin level
increased significantly in women with PCOS compared to controls in all age and BMI groups.
Conclusion: A significant decrease in ghrelin and an increase in leptin in PCOS patients were observed
than in controls, indicating that they are at high risk for metabolic syndrome development.
