Login Register Cart 0
Generic placeholder image

Anti-Cancer Agents in Medicinal Chemistry

Editor-in-Chief

ISSN (Print): 1871-5206
ISSN (Online): 1875-5992

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Mechanistic Study on Thymoquinone Conjugated ZnO Nanoparticles Mediated Cytotoxicity and Anticancer Activity in Triple-Negative Breast Cancer Cells

Author(s): Sampath K. Banupriya, Krishnamoorthy Kavithaa, Arumugam Poornima and Sundaravadivelu Sumathi*

Volume 22, Issue 2, 2022

Published on: 12 April, 2021

Page: [313 - 327] Pages: 15

DOI: 10.2174/1871520621666210412104731

Price: $65

Abstract

Background: In the current era, the development of molecular techniques involves nano techniques, and the synthesis of nanoparticles is considered the preferred field in nanotechnology.

Objective: The aim of the present work is to analyze the anticancer activity of the thymoquinone conjugated ZnO nanoparticles and understand its mechanism of action in triple-negative breast cancer cell lines MDA-MB-231.

Methods: Zinc Oxide (ZnO) nanoparticles have extensive applications, and it was synthesized using a chemical precipitation method. Thymoquinone (TQ) is the major bioactive component of the seeds of Nigella sativa. Synthesized nanoparticles were characterized using various spectroscopic techniques. Thymoquinone-coated nanoparticles were checked for their efficiency. The cytotoxicity of ZnO, TQ, and TQ conjugated ZnO nanoparticles against MDA-MB-231. Colony-forming and cell migration assays were performed to measure the proliferative competence of the breast cancer cells on exposure to nanoparticles. The mechanism of apoptosis was probed by assessing MMP, interplay between ER stress and ROS.

Results: The results of the characterization techniques confirmed that the particles synthesized were ZnO and TQ-ZnO nanoparticles. pH dependent release of the compound was observed. The anti-proliferative effect that impairs the formation of the colony was found to be enhanced in cells exposed to combined treatment with the nanoconjugate.

Conclusion: Hence, the TQ conjugated ZnO nanoparticles can act as an efficient carrier for drug delivery at the target site in TNBC cells.

« Previous Next »
Graphical Abstract

[1]
Tran, P.; Lee, S.E.; Kim, D.H.; Pyo, Y.C.; Park, J.S. Recent advances of nanotechnology for the delivery of anticancer drugs for breast cancer treatment. J. Pharm. Investig., 2020, 50(3), 261-270.
[http://dx.doi.org/10.1007/s40005-019-00459-7]
[2]
Qin, J.; Lian, J.; Wu, S.; Wang, Y.; Shi, D. Recent advances in nanotechnology for breast cancer therapy.Nano Life, 2019, 9(2), 01n02.,
[http://dx.doi.org/10.1142/S1793984419400038]
[3]
Bharat, T.C. Shubham, Mondal,S.; Gupta, H.S.; Singh, P.K.; Das, A.K. Synthesis of doped zinc oxide nanoparticles: A review. Materials Today: Proceedings, 2019, 11, 767-775.
[4]
Chai, H.Y.; Lam, S.M.; Sin, J.C. Green synthesis of magnetic Fe-doped ZnO nanoparticles via Hibiscus rosa-sinensis leaf extracts for boosted photocatalytic, antibacterial and antifungal activities. Mater. Lett., 2019, 241, 103-106.
[http://dx.doi.org/10.1016/j.matlet.2019.01.116]
[5]
Ranganathan, V.L.; Nithin, K.S.; Khanum, S.A.; Nagaraju, G.; Mallikarjunaswamy, C. Zinc oxide nanoparticles: A significant review on synthetic strategies, characterization and applications AIP Conference Proceedings, 2019, 2162;
[http://dx.doi.org/10.1063/1.5130299]
[6]
Odeh, F.; Naffa, R.; Azzam, H.; Mahmoud, I.S.; Alshaer, W.; Al Bawab, A.; Ismail, S. Co-encapsulation of thymoquinone with docetaxel enhances the encapsulation efficiency into PEGylated liposomes and the chemosensitivity of MCF7 breast cancer cells to docetaxel. Heliyon, 2019, 5(11), e02919-e02925.
[http://dx.doi.org/10.1016/j.heliyon.2019.e02919] [PMID: 31844767]
[7]
Sundaravadivelu, S.; Raj, S.K.; Kumar, B.S.; Arumugamand, P.; Ragunathan, P.P. Reverse screening bioinformatics approach to identify potential anti breast cancer targets using thymoquinone from neutraceuticals black cumin oil. Anticancer. Agents Med. Chem., 2019, 19(5), 599-609.
[http://dx.doi.org/10.2174/1871520619666190124155359] [PMID: 30678630]
[8]
Banupriya, S.J.S.; Kavithaa, K.; Poornima, A.; Sumathi, S. Synthesis, characterization and cytotoxicity of thymoquinone conjugated ZnO nanoparticles in triple negative breast cancer cells. Scrutiny Int. Res. J. Biolog. Environ. Sci., 2020, 7, 1-8.
[9]
Khan, M.A.; Tania, M.; Fu, J. Epigenetic role of thymoquinone: impact on cellular mechanism and cancer therapeutics. Drug Discov. Today, 2019, 24(12), 2315-2322.
[http://dx.doi.org/10.1016/j.drudis.2019.09.007] [PMID: 31541714]
[10]
Ballout, F.; Habli, Z.; Rahal, O.N.; Fatfat, M.; Muhtasib, H.G. Thymoquinone-based nanotechnology for cancer therapy: promises and challenges. Drug Discov. Today, 2018, 23(5), 1089-1098.
[http://dx.doi.org/10.1016/j.drudis.2018.01.043]
[11]
Xu, C.; Thiruvadi, V.S.; Whitmore, R.; Liu, H. Delivery systems for biomedical applications: Basic introduction, research frontier and clinical translations. Biomed. Transl. Med., 2019, 93-116.,
[http://dx.doi.org/10.1016/B978-0-12-813477-1.00005-0]
[12]
Reddy, N.; Reddy, C.V.; Sreedhar, M.; Shim, J.; Cho, M.; Kim, D. Effect of ball milling on optical properties and visible photocatalytic activity of Fe doped ZnO nanoparticles. Mater. Sci. Eng. B, 2019, 240, 33-40.
[http://dx.doi.org/10.1016/j.mseb.2019.01.002]
[13]
Auger, S.; Henry, C.; Pechaux, C. Exploring the impact of Mg-doped ZnO nanoparticles on a model soil microorganism Bacillus subtilus; Ecotox Environ Safe, 2019, p. 182.
[http://dx.doi.org/10.1016/j.ecoenv.2019.109421]
[14]
Jamdagni, P.; Khatri, P.; Rana, J.S. Green synthesis of zinc oxide nanoparticles using flower extract of Nyctanthes arbor-tristis and their antifungal activity. J.A King Saud. Univ. Sci., 2018, 30(2), 168-175.
[http://dx.doi.org/10.1016/j.jksus.2016.10.002]
[15]
Yusof, N.M.A.; Zain, N.M. Synthesis of ZnO nanoparticles with chitosan as stabilizing agent and their antibacterial properties against Gram-positive and Gram-negative bacteria. Int. J. Biol. Macromol., 2019, 124, 1132-1136.
[http://dx.doi.org/10.1016/j.ijbiomac.2018.11.228]
[16]
Kavithaa, K.; Paulpandi, M.; Padma, P.R.; Sumathi, S. Induction of intrinsic apoptotic pathway and cell cycle arrest via baicalein loaded iron oxide nanoparticles as a competent nano-mediated system for triple negative breast cancer therapy. RSC Advances, 2016, 6(69), 64531-64543.
[http://dx.doi.org/10.1039/C6RA11658B]
[17]
Sadhukhan, P.; Kundu, M.; Rana, S.; Kumar, R.; Das, J.; Sil, P.C. Microwave induced synthesis of ZnO nanorods and their efficacy as a drug carrier with profound anticancer and antibacterial properties. Toxicol. Rep., 2019, 6, 176-185.
[http://dx.doi.org/10.1016/j.toxrep.2019.01.006]
[18]
Wang, H.; Gong, X.; Guo, X.; Liu, C.; Fan, Y.Y.; Zhang, J.; Niu, B.; Li, W. Characterization, release, and antioxidant activity of curcumin-loaded sodium alginate/ZnO hydrogel beads. Int. J. Biol. Macromol., 2019, 121, 1118-1125.
[http://dx.doi.org/10.1016/j.ijbiomac.2018.10.121] [PMID: 30340010]
[19]
Mou, J.; Liu, Z.; Liu, J.; Lu, J.; Zhu, W.; Pei, D. Hydrogel containing minocycline and zinc oxide-loaded serum albumin nanopartical for periodontitis application: preparation, characterization and evaluation. Drug Deliv., 2019, 26(1), 179-187.
[http://dx.doi.org/10.1080/10717544.2019.1571121] [PMID: 30822158]
[20]
Sabzichi, M.; Ramezani, M.; Mohammadian, J. The synergistic impact of quinacrine on cell cycle and anti-invasiveness behaviors of doxorubicin in MDA-MB-231 breast cancer cells. Process Biochem., 2019, 81, 175-181.
[http://dx.doi.org/10.1016/j.procbio.2019.03.007]
[21]
Chowdhury, P.; Nagesh, P.K.B.; Hatami, E.; Wagh, S.; Dan, N.; Tripathi, M.K.; Khan, S.; Hafeez, B.B.; Meibohm, B.; Chauhan, S.C.; Jaggi, M.; Yallapu, M.M. Tannic acid-inspired paclitaxel nanoparticles for enhanced anticancer effects in breast cancer cells. J. Colloid Interface Sci., 2019, 535, 133-148.
[http://dx.doi.org/10.1016/j.jcis.2018.09.072] [PMID: 30292104]
[22]
Jia, T.T.; Yang, G.; Mo, S.J.; Wang, Z.Y.; Li, B.J.; Ma, W.; Guo, Y.X.; Chen, X.; Zhao, X.; Liu, J.Q.; Zang, S.Q. Atomically precise gold-levonorgestrel nanocluster as a radiosensitizer for enhanced cancer therapy. ACS Nano, 2019, 13(7), 8320-8328.
[http://dx.doi.org/10.1021/acsnano.9b03767] [PMID: 31241895]
[23]
Aonal, T.D.; Hamurcu, Z.; Delibas, N.; Cinar, V.; Guler, A.; Gokce, S.; Ozpolat, B. Thymoquinone inhibits proliferation and migration of MDAMB- triple negative breast cancer cells by suppressing autophagy and beclin-1 and LC3. Anticancer. Agents Med. Chem., 2020. [Online ahead of print.].
[PMID: 32767958]
[24]
Shaniba, V.S.; Aziz, A.A.; Jayasree, P.R.; Kumar, P.R.M. Manilkara zapota (L.) P. Royen leaf extract derived silver nanoparticles induce apoptosis in human colorectal carcinoma cells without affecting human lymphocytes or erythrocytes. Biol. Trace Elem. Res., 2019, 192(2), 160-174.
[http://dx.doi.org/10.1007/s12011-019-1653-6] [PMID: 30850949]
[25]
Iglesias-Figueroa, B.F.; Siqueiros-Cendon, T.S.; Gutierrez, D.A. Recombinant human lactoferrin induces apoptosis, disruption of F-actin structure and cell cycle arrest with selective cytotoxicity on human triple negative breast cancer cells. Apoptosis, 2019, 24(7-8), 562-577.
[http://dx.doi.org/10.1007/s10495-019-01539-7] [PMID: 30941553]
[26]
Huang, W.; Liang, Y.; Ma, X. Alpha-mangostin induces endoplasmic reticulum stress and autophagy which count against fatty acid synthase inhibition mediated apoptosis in human breast cancer cells. Cancer Cell Int., 2019, 19(1), 151-159.
[http://dx.doi.org/10.1186/s12935-019-0869-z] [PMID: 31164796]
[27]
Sadhukhan, P.; Kundu, M.; Chatterjee, S.; Ghosh, N.; Manna, P.; Das, J.; Sil, P.C. Targeted delivery of quercetin via pH-responsive zinc oxide nanoparticles for breast cancer therapy. Mater. Sci. Eng. C, 2019, 100, 129-140.
[http://dx.doi.org/10.1016/j.msec.2019.02.096] [PMID: 30948047]

Rights & Permissions Print Cite
Article Metrics
41
4
Wayfinder Image
© 2024 Bentham Science Publishers | Privacy Policy