Title:An Optimal Combination of Chemically Pure Compounds from Salvia miltiorrhiza
for Inhibiting Cell Proliferation
Volume: 20
Issue: 1
Author(s): Tiemei Shao, Jing Zhang, Shengjun An*, Hongjun Xu, Jiangli Wu, Lei Wang, Wei Zhao, Yongjie Zhou, Luyang Zhou and Qingzhuo Cui
Affiliation:
- Hebei Engineering Center of Plant Bioreactor Preparation Technology, Hebei University of Chinese Medicine, Shijiazhuang,
China
- Research Center,
Hebei University of Chinese Medicine, Shijiazhuang, China
- College of Integrated Chinese and Western Medicine, Hebei
University of Chinese Medicine, Shijiazhuang, China
Keywords:
Aqueous extract of salvia, uniform design, orthogonal design, formulation, oxidative stress, herb medicine.
Abstract:
Objective: Salvia miltiorrhiza (SM) is a traditional Chinese medicine used clinically to
treat cardiovascular diseases, including atherosclerosis and myocardial infarction. Its therapeutic effect
has been confirmed by many clinical and pharmacological studies. However, the optimal formulation
of active ingredients in SM for treating cardiovascular diseases remains unclear. In this
study, we determined the ratio of the optimal compatibility of SM ingredients DSS, Sal-A, Sal-B,
and PAL (SABP)with a uniform and orthogonal optimized experimental design. In addition, we determined
the anti-oxidation effect of SABP using Adventitial Fibroblasts (AFs).
Methods: By using a combination of uniform and orthogonal designs, we determined the optimal
formulation of aqueous extract from SM. MTT assay was used to determine the inhibitory effects
of these 4 components of SM on the AFs, which were isolated and cultured from the aorta. The reactive
oxygen species (ROS) production in AFs was compared before and after SABP treatment.
Results: The optimal formulation of these 4 aqueous extracts from SM were 150 : 7 : 300 : 500,
and their concentrations were S(1.5×10-4 mol/L), A(7×10-6 mol/L), B(3×10-4 mol/L), and
P(5×10-4 mol/L). There were some synergies between these 4 components. Moreover, SABP decreased
ROS production in AFs.
Conclusion: These findings suggest that SABP inhibits the proliferation and oxidation stress in
AFs. The present study provides new evidence that the efficacy and function generated from the optimal
formulation of active ingredients in SM are better than lyophilized powder of SM.
