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Current Pharmaceutical Biotechnology

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ISSN (Print): 1389-2010
ISSN (Online): 1873-4316

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Research Article

Formulations for Effective Detoxification Derived from Three Medicinal Plants: Thunbergia laurifolia, Clerodendrum disparifolium and Rotheca serrata

Author(s): Prasit Boonthai, Kowit Noikotr, Naraphatra Saemram, Runglawan Sudmoon, Tawatchai Tanee, Arunrat Chaveerach, Natcha Patarapadungkit and Pornnarong Siripiyasing*

Volume 23, Issue 1, 2022

Published on: 08 February, 2021

Page: [140 - 147] Pages: 8

DOI: 10.2174/1389201022666210208145605

Price: $65

Abstract

Background: Based on the long history of the medicinal use of Thunbergia laurifolia, Clerodendrum disparifolium and Rotheca serrata, the extract formulations of these species: T. laurifolia and C. disparifolium; T. laurifolia and R. serrata; and T. laurifolia, C. disparifolium and R. serrata, called formulas 1, 2 and 3, were created for detoxification testing to take more advantage of each species.

Objective: The objective of this study is to estimate the detoxifying effects of studied extract formulations on human cell and tissue culture as a preclinical trial.

Methods: The major phytochemicals were derived by GC-MS. The detoxification efficacy of these formulations in cells and DNA levels were derived by MTT and comet assays in toxic PBMCs (incubated with rice whisky or bathroom cleaner).

Results: The phytochemical constituents were detected at 23.48% phytol and 43.03% oleamide in T. laurifolia; 12.88% oleamide, 20.93% 9,12,15-octadecatrien, 25.52% squalene, 22.19% butylated hydroxy toluene and 15.36% vitamin E in C. disparifolium; and 30.41% phytol, 32.78% oleamide, and 12.20%, 9,12,15-octadecatrien-1-ol in R. serrata. The toxic cells treated with the plant formulas 1, 2 and 3 showed no IC50 values, but formulas 1 and 2 displayed higher efficacies than formula 3 did. The comet assay indicated that the experiments (the treatment on toxic cells with the plant formulas) induced significant (p < 0.05) DNA damage compared to the negative control due to poisoning occurring before administration of the plant formulas. The OTM of the controls was significantly (p < 0.05) longer than the experimental samples showing significantly reduce the toxicity of the created formulations.

Conclusion: The formulas showed high detoxification efficacies and formulations 1 and 2 resulted in higher levels of detoxification than formulation 3, especially in immediate treatment after receiving toxic substances.

Keywords: Thunbergia laurifolia, Clerodendrum disparifolium, Rotheca serrata, detoxification, OTM, phytochemical constituents.

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