Title:Electro-Acupuncture Protects Diabetic Nephropathy-Induced Inflammation Through Suppression of NLRP3 Inflammasome in Renal Macrophage
Isolation
Volume: 21
Issue: 11
Author(s): Jieying Zhang, Xiao Yang, Xinhuan Zhang, Dan Lu and Rui Guo*
Affiliation:
- Department of Accupuncture, Zibo Integrated Traditional Chinese and Western Medicine Hospital, Tianjin 300381,China
Keywords:
Electro-acupuncture, diabetic nephropathy, inflammasome, HMGB1, NLRP3, diabetic microangiopathy.
Abstract:
Background: With the increasing prevalence of diabetes in recent years, diabetic nephropathy
(DN) has also become a dangerous disease that greatly endangers human health. The study
was designed to determine the mechanism and effect of Electro-acupuncture (EA) in alleviating
DN-induced inflammation.
Methods: Mice were intraperitoneally injected with STZ (streptozotocin, 50 mg/kg, S0130, Sigma,
St. Louis, MO, USA) for five consecutive days. After 12 weeks of induction, blood glucose levels
were measured (>300 mg/dL).
Results: The serum levels of IL-1β and IL-6 were decreased in EA-treated DN mice, and EA protected
renal injury. EA could suppress HMGB1/NLRP3/NF-κB pathway. HMGB1 inhibition increased
the anti-inflammatory effects of EA on DN by suppressing NLRP3/NF-κB pathway. The
activation of HMGB1 attenuated the anti-inflammatory effects of EA on DN by inducing NLRP3/
NF-κB pathway.
Conclusion: These results suggested that EA protected DN-induced inflammation through the suppression
of NLRP3 inflammasome.
