Title:Phytochemical Screening and In-Vitro Antibacterial and Anticancer Activity of Crude Extract of Matricaria aurea
Volume: 27
Issue: 1
Author(s): Irfan Ahmad, Mushtaq Ahmad Mir, Saurabh Srivastava*, Ali A. Shati, Serag Eldin I. Elbehairi, Safia Irfan, Mohammed Abohashrh, Nazima Nisar, Nasreena Bashir and Pragya Srivastava
Affiliation:
- Department of Pharmaceutics, Era College of Pharmacy, Era University, Lucknow, UP,India
Keywords:
M. aurea, anticancer activity, antibacterial activity, antibiotics, inhibition zone, crude extract.
Abstract:
Background: Infectious diseases constantly represent the source of sickness as well as mortality in
human beings. Herbal applications in human life through using plants for antibacterial and anticancer activity
have shown the potential medicinal outcome.
Objectives: To evaluate the antibacterial and anticancer activities of the crude extract of Matricaria aurea.
Materials and Methods: The antibacterial activity of the crude flowers of M. aurea extract was examined
against reference and clinical bacterial strains by agar well diffusion method. Minimum inhibitory concentrations
and minimum bactericidal concentrations were determined by micro broth dilution assays using MH
broth. Herbal extract was employed over human breast adenocarcinoma cell line (MCF-7), hepatocellular carcinoma
cell line (HepG-2) and colorectal adenocarcinoma cell line (HCT-116) to optimize cancer cells proliferation
by SRB assay.
Results: The data has shown that the extract from M. aurea had significant antimicrobial activity against the
tested microorganisms. The plant extract showed higher antibacterial activity against the reference strain of
Streptococcus pyogenes. The MIC and MBC varied between 0.38-12.5 mg/ml and 3.1-200 mg/ml respectively.
Synergy study elucidated the significant bacteriostatic effect of M. aurea extract on S. aureus and S. saprophyticus.
The data of SRB assay deliver the potential anticancer activity through cell death.
Conclusion: This study delivers innovative information that M. aurea possessed excellent bio-activities against
pathogenic microbes and cancer cells, which drive attention for further research to explore the active components
responsible for biological efficacies.
