Title:Modulation of MicroRNAs by Euphorbia Microsciadia Boiss in MDA-MB-231 Cell Line: New Possibilities in Breast Cancer Therapy
Volume: 15
Issue: 2
Author(s): Mohammad-Reza Mahmoudian-Sani and Majid Asadi-Samani*
Affiliation:
- Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord,Iran
Keywords:
Apoptosis, breast cancer, cell cycle, euphorbia, microRNA, miR-34a, tumor suppressor.
Abstract:
Background: A large number of Euphorbia species have been evaluated for anticancer
effects; however, their anticancer mechanisms have not been established up to now.
Objective: The present study aimed to evaluate the effects of Euphorbia microsciadia (E. microsciadia)
Boiss on the modulation of micro (mi) RNAs in MDA-MB-231 cell line.
Methods: As the first step, the inhibitory concentration of hydroalcoholic extract of E. microsciadia
on MDA-MB-231 cells was examined using the MTT assay, bypassing 24 and 48h from seeding.
The real-time quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) was
also utilized to determine Let-7, miR-15, miR-16, miR-29, miR-151, miR-155, miR-21, miR-146b,
miR-181b, miR-221, miR-222, miR-21, and miR-146b expressions in MDA-MB-231 cells, by passing
24 and 48h from treating with the extract of E. microsciadia.
Results: The results reveal the cytotoxic effects of E. microsciadia on MDA-MB-231 cell line in a
dose-dependent manner. The half maximal Inhibitory Concentrations (IC50) were also equal to
275 and 240μg/ml for E. microsciadia, by passing 24 and 48h from the treatment, respectively. Furthermore,
it was confirmed that, E. microsciadia had augmented the expression levels of Let-7,
miR-15, miR-16, miR-29, and miR-34a, which lead to an increase in apoptosis.
Conclusion: E. microsciadia could modulate some miRNAs involved in cell cycle arrest and apoptosis
in MDA-MB-231 cell line. Accordingly, targeting miRNAs by E. microsciadia can open
some newer avenues for breast cancer therapy.
