Title:Roux-en-Y Gastrointestinal Bypass Promotes Activation of TGR5 and Peptide YY
Volume: 20
Issue: 8
Author(s): Haojun Yang, Hanyang Liu, YuWen Jiao and Jun Qian*
Affiliation:
- Department of Gastrointestinal Surgery, Nanjing Medical University Affiliated Changzhou No. 2 People’s Hospital, Changzhou, 213000,China
Keywords:
Roux-en-Y, gastrointestinal bypass, obesity, TGR5, PYY, high-fat diet.
Abstract:
Background: G protein-coupled bile acid receptor (TGR5) is involved in a number of metabolic
diseases. The aim of this study was to identify the role of TGR5 after Roux-en-Y gastric bypass
(GBP).
Methods: Wild type and TGR5 knockout mice (tgr5-/-) were fed a high-fat diet (HFD) to establish the
obesity model. GBP was performed. The changes in body weight and food intake were measured. The
levels of TGR5 and peptide YY (PYY) were evaluated by RT-PCR, Western blot, and ELISA. Moreover,
the L-cells were separated from wild type and tgr5-/- mice. The levels of PYY in L-cells were
evaluated by ELISA.
Results: The body weights were significantly decreased after GBP in wild type mice (p<0.05), but not
tgr5-/- mice (p>0.05). Food intake was reduced after GBP in wild type mice, but also not significantly
affected in tgr5-/- mice (p>0.05). The levels of PYY were significantly increased after GBP compared
with the sham group (p<0.05); however, in tgr5-/- mice the expression of PYY was not significantly
affected (p>0.05). After INT-777 stimulation in L-cells obtained from murine intestines, the levels of
PYY were significantly increased in L-cells tgr5+/+ (p<0.05).
Conclusion: Our study suggests that GBP up-regulated the expression of TGR5 in murine intestines,
and increased the levels of PYY, which further reduced food intake and decreased the body weight.
