Title:Integrated Analysis of mRNA-seq and miRNA-seq to Identify c-MYC, YAP1 and miR-3960 as Major Players in the Anticancer Effects of Caffeic Acid Phenethyl Ester in Human Small Cell Lung Cancer Cell Line
Volume: 20
Issue: 1
Author(s): Fei Mo, Ya Luo, Dian Fan, Hao Zeng, Yunuo Zhao, Meng Luo, Xiaobei Liu and Xuelei Ma*
Affiliation:
- Laboratory of Tumor Targeted and Immune Therapy, Clinical Research Center for Breast, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, Sichuan,China
Keywords:
Caffeic acid phenethyl ester, small cell lung cancer, miR-3960, c-MYC, YAP1, high-throughput sequencing.
Abstract:
Background: Caffeic Acid Phenethyl Ester (CAPE), an active extract of propolis, has recently
been reported to have broad applications in various cancers. However, the effects of CAPE on
Small Cell Lung Cancer (SCLC) are largely unknown. Therefore, the aim of this study was to determine
the anti-proliferative effect of CAPE and explore the underlying molecular mechanisms in SCLC
cells using high-throughput sequencing and bioinformatics analysis.
Methods: Small-cell lung cancer H446 cells were treated with CAPE, and cell proliferation and apoptosis
were then assessed. Additionally, the regulation mediated by miR-3960 after CAPE treatment
was explored and the altered signaling pathways were predicted in a bioinformatics analysis.
Results: CAPE significantly inhibited cell proliferation and induced apoptosis. CAPE decreased the
expression of Yes-Associated Protein 1 (YAP1) and cellular myelocytomatosis oncogene (c-MYC)
protein. Moreover, the upregulation of miR-3960 by CAPE contributed to CAPE-induced apoptosis.
The knockdown of miR-3960 decreased the CAPE-induced apoptosis.
Conclusion: We demonstrated the anti-cancer effect of CAPE in human SCLC cells and studied the
mechanism by acquiring a comprehensive transcriptome profile of CAPE-treated cells.
