Login Register Cart 0
Generic placeholder image

Current Bioactive Compounds

Editor-in-Chief

ISSN (Print): 1573-4072
ISSN (Online): 1875-6646

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Prevention and Healing of Calcium Signaling Mediated Neuronal Damage on successive Administration of Flavonoid Enriched Pterocarpus Marsupium Roxb in Peripheral Neuropathy Model

Author(s): Neethi Shaju, Mrinmoy Gautam, Abdul Khayum and Gunasekaran Venkatesh*

Volume 16, Issue 9, 2020

Page: [1346 - 1355] Pages: 10

DOI: 10.2174/1573407216666200218112305

Price: $65

Open Access Journals Promotions 2
Abstract

Background: Modern research on peripheral neuropathy circumstance utter that treatments with Vincristine (VCR) disturb the microtubular cells in sensory and motor neurons due to calcium over- load in sciatic nerve, unfortunately, VCR triggering the release of Tumor necrosis factor-α (TNFα) in central neurons causes excitotoxicity as well. Although ethnomedical information specifies that Pterocarpus marsupium Roxb (PM) is widely used for various nervous disorders, not yet justified on VCR induced peripheral neuropathy and in relation to central mechanism.

Objective: This study is aimed to explore the possible central and peripheral mechanism of flavonoid enriched PM in VCR induced neuropathy model.

Methods: Neuropathic pain was induced in female Wistar rats by VCR (75μg/ kg/day, i.p) for 10 days. Nociceptive thresholds were assessed by subjecting them to behavioral and biochemical estimation, proinflammatory cytokines along with morphological evaluation.

Results: PM significantly increased the nociceptive threshold evident from various behavioral models in comparison to VCR group. More importantly, PM significantly reversed the VCR induced calcium elevation, glutamate and aspartate release in the brain.

Discussion: It was also observed that the raised TNF-α, Interleukin-1β were controlled and interleukin- 10 was elevated in sciatic nerve after PM treatment. Evident from histology, PM markedly reversed the VCR induced axonal degeneration, Schwann cell hyperplasia, and myelin fibrosis.

Conclusion: Flavonoid enriched PM both 100 & 200mg/kg post and co-administration exerted a preventive and curative effect in VCR induced neuropathic pain by controlling calcium-mediated excitotoxicity through peripheral and central mechanism.

Keywords: Vincristine, flavanoids, calcium, glutamate, central action, peripheral neuropathy.

Graphical Abstract

[1]
Jensen, T.S.; Finnerup, N.B. Allodynia and hyperalgesia in neuropathic pain: Clinical manifestations and mechanisms. Lancet Neurol., 2014, 13(9), 924-935.
[http://dx.doi.org/10.1016/S1474-4422(14)70102-4] [PMID: 25142459]
[2]
Alston, R.P.; Pechon, P. Dysaesthesia associated with sternotomy for heart surgery presented at the association of cardiothoracic anaesthe-tists meeting, november 2003, institute of electrical engineers, London and the world congress of anaesthesiologists, Paris, 21st April, 20. Br. J. Anaesth., 2005, 95, 153-158.
[http://dx.doi.org/10.1093/bja/aei152] [PMID: 15894562]
[3]
Brewer, J.R.; Morrison, G.; Dolan, M.E.; Fleming, G.F. Chemotherapy-induced peripheral neuropathy: Current status and progress. Gynecol. Oncol., 2016, 140(1), 176-183.
[http://dx.doi.org/10.1016/j.ygyno.2015.11.011] [PMID: 26556766]
[4]
Jaggi, A.S.; Jain, V.; Singh, N. Animal models of neuropathic pain. Fundam. Clin. Pharmacol., 2011, 25(1), 1-28.
[http://dx.doi.org/10.1111/j.1472-8206.2009.00801.x] [PMID: 20030738]
[5]
Kesik, V.; Kurt, B.; Tunc, T.; Karslioglu, Y.; Citak, E.C.; Kismet, E.; Koseoglu, V. Adrenomedullin worsens skin necrosis in rats subjected to vincristine-induced extravasation. Clin. Exp. Dermatol., 2010, 35(8), 897-901.
[http://dx.doi.org/10.1111/j.1365-2230.2010.03803.x] [PMID: 20345993]
[6]
Thiagarajan, V.R.K.; Shanmugam, P.; Krishnan, U.M.; Muthuraman, A. Ameliorative effect of Vernonia cinerea in vincristine-induced painful neuropathy in rats. Toxicol. Ind. Health, 2014, 30(9), 794-805.
[http://dx.doi.org/10.1177/0748233712463779] [PMID: 23081859]
[7]
Ghelardini, C.; Desaphy, J.F.; Muraglia, M.; Corbo, F.; Matucci, R.; Dipalma, A.; Bertucci, C.; Pistolozzi, M.; Nesi, M.; Norcini, M.; Franchini, C.; Camerino, D.C. Effects of a new potent analog of tocainide on hNav1.7 sodium channels and in vivo neuropathic pain models. Neuroscience, 2010, 169(2), 863-873.
[http://dx.doi.org/10.1016/j.neuroscience.2010.05.019] [PMID: 20580661]
[8]
Joseph, E.K.; Levine, J.D. Caspase signalling in neuropathic and inflammatory pain in the rat. Eur. J. Neurosci., 2004, 20(11), 2896-2902.
[http://dx.doi.org/10.1111/j.1460-9568.2004.03750.x] [PMID: 15579143]
[9]
Muthuraman, A.; Diwan, V.; Jaggi, A.S.; Singh, N.; Singh, D. Ameliorative effects of Ocimum sanctum in sciatic nerve transection-induced neuropathy in rats. J. Ethnopharmacol., 2008, 120(1), 56-62.
[http://dx.doi.org/10.1016/j.jep.2008.07.049] [PMID: 18762236]
[10]
Ellis, R.J.; Toperoff, W.; Vaida, F.; van den Brande, G.; Gonzales, J.; Gouaux, B.; Bentley, H.; Atkinson, J.H. Smoked medicinal cannabis for neuropathic pain in HIV: A randomized, crossover clinical trial. Neuropsychopharmacology, 2009, 34(3), 672-680.
[http://dx.doi.org/10.1038/npp.2008.120] [PMID: 18688212]
[11]
Joshi, K.S.; Bhonde, R. Insights from Ayurveda for translational stem cell research. J. Ayurveda Integr. Med., 2014, 5(1), 4-10.
[http://dx.doi.org/10.4103/0975-9476.128846] [PMID: 24812469]
[12]
Pant, D.R.; Pant, N.D.; Saru, D.B.; Yadav, U.N.; Khanal, D.P. Phytochemical screening and study of antioxidant, antimicrobial, antidiabetic, anti-inflammatory and analgesic activities of extracts from stem wood of Pterocarpus marsupium Roxburgh. J. Intercult. Ethnopharmacol., 2017, 6(2), 170-176.
[http://dx.doi.org/10.5455/jice.20170403094055] [PMID: 28512598]
[13]
Hugar, A.L.; Kanjikar, A.P.; Londonkar, R.L. A novel potential reproductive effects of Pterocarpus marsupium methanolic extract on testosterone propionate induced polycystic ovary syndrome in female albino rats. Endocr. Metab. Immune Disord. Drug Targets, 2017, 17(4), 317-323.
[http://dx.doi.org/10.2174/1871530317666170912153934] [PMID: 28901866]
[14]
Badkhane, Y.; Yadav, A.S.; Sharma, A.K.; Raghuwanshi, D.K.; Uikey, S.K.; Mir, F.A.; Lone, S.A.; Murab, T. Pterocarpus marsupium Roxb - Biological activities and medicinal properties. Int. J. Adv. Pharm. Sci., 2010, 2010(1), 4.
[15]
Joshi, K.R.; Devkota, H.P.; Yahara, S. Chemical Analysis of Heartwood of Bijayasal (Pterocarpus marsupium Roxb.). Nepal J. Sci. Technol., 2013, 13(2), 219-224.
[http://dx.doi.org/10.3126/njst.v13i2.7740]
[16]
Randall, L.O.; Selitto, J.J. A method for measurement of analgesic activity on inflamed tissue. Arch. Int. Pharmacodyn. Ther., 1957, 111(4), 409-419.
[PMID: 13471093]
[17]
Chaplan, S.R.; Bach, F.W.; Pogrel, J.W.; Chung, J.M.; Yaksh, T.L. Quantitative assessment of Tactile allodynia in the rat paw. J. Neurosci. Methods, 1994, 53(1), 55-63.
[http://dx.doi.org/10.1016/0165-0270(94)90144-9] [PMID: 7990513]
[18]
Meunier, C.J.; Burton, J.; Cumps, J.; Verbeeck, R.K. Evaluation of the formalin test to assess the analgesic activity of diflunisal in the rat. Eur. J. Pharm. Sci., 1998, 6(4), 311-316.
[http://dx.doi.org/10.1016/S0928-0987(97)10020-3] [PMID: 9795087]
[19]
Iohom, G.; Lan, G.B.; Diarra, D.P.; Grignon, Y.; Kinirons, B.P.; Girard, F.; Merle, M.; Granier, G.; Cahn, V.; Bouaziz, H. Long-term evaluation of motor function following intraneural injection of ropivacaine using walking track analysis in rats. Br. J. Anaesth., 2005, 94(4), 524-529.
[http://dx.doi.org/10.1093/bja/aei079] [PMID: 15695548]
[20]
Gautam, M.; Ramanathan, M. Saponins of Tribulus terrestris attenuated neuropathic pain induced with vincristine through central and peripheral mechanism. Inflammopharmacology, 2019, 27(4), 761-772.
[http://dx.doi.org/10.1007/s10787-018-0502-0] [PMID: 29938333]
[21]
Babu, C.S.; Sunil, A.G.; Vasanthi, H.R.; Muthusamy, V.S.; Ramanathan, M. Development and validation of an HPTLC method for simultaneous estimation of excitatory neurotransmitters in rat brain. J. Liq. Chromatogr. Relat. Technol., 2007, 30(19), 2891-2902.
[http://dx.doi.org/10.1080/10826070701588760]
[22]
Severinghaus, J.W.; Ferrebee, J.W. Calcium determination by flame photometry; methods for serum, urine, and other fluids. J. Biol. Chem., 1950, 187(2), 621-630.
[PMID: 14803444]
[23]
Thiagarajan, V.R.K.; Shanmugam, P.; Krishnan, U.M.; Muthuraman, A. Ameliorative potential of Vernonia cinerea on chronic constriction injury of sciatic nerve induced neuropathic pain in rats. An. Acad. Bras. Cienc., 2014, 86(3), 1435-1450.
[http://dx.doi.org/10.1590/0001-3765201420130404] [PMID: 25211113]
[24]
Sen, O.; Sayilgan, N.C.; Tutuncu, A.C.; Bakan, M.; Koksal, G.M.; Oz, H. Evaluation of sciatic nerve damage following intraneural injection of bupivacaine, levobupivacaine and lidocaine in rats. Braz. J. Anesthesiol., 2016, 66(3), 272-275.
[http://dx.doi.org/10.1016/j.bjane.2014.09.012] [PMID: 27108824]
[25]
Geisler, S.; Doan, R.A.; Strickland, A.; Huang, X.; Milbrandt, J.; DiAntonio, A. Prevention of vincristine-induced peripheral neuropathy by genetic deletion of SARM1 in mice. Brain, 2016, 139(Pt 12), 3092-3108.
[http://dx.doi.org/10.1093/brain/aww251] [PMID: 27797810]
[26]
Krishna, V.; Manjunatha, B.; Vidya, S.; Jagadeesh Singh, S.; Manohara, Y.; Raheman, A-U.; Avinash, K.; Mankani, K. Evaluation of hepa-toprotective activity of stem bark of Pterocarpus marsupium Roxb. Indian J. Pharmacol., 2005, 37(3), 165.
[http://dx.doi.org/10.4103/0253-7613.16213]
[27]
Chawla, A.; Kaur, J.; Sharma, A.K. Systemic review: Pharmacognosy, phytochemistry, pharmacology and clinical applications of Pterocarpus marsupium Roxb. Int. J. Pharm. Phytopharm. Res, 2013, 2(5), 319-327.
[28]
Devgun, N.A.; Ansari, S. Pterocarpus marsupium Roxb. - A comprehensive review. Pharmacogn. Rev., 2009, 3(6), 359-363.
[29]
Ishii, N.; Matsuoka, Y.; Omiya, H.; Taniguchi, A.; Kaku, R.; Morita, K. The flavonoid quercetin suppreses the development of neuropathic pain behavior in rats. Eur. J. Anaesthesiol., 2013, 30(1), 214-214.
[http://dx.doi.org/10.1097/00003643-201306001-00667]
[30]
Field, M.J.; McCleary, S.; Hughes, J.; Singh, L. Gabapentin and pregabalin, but not morphine and amitriptyline, block both static and dynamic components of mechanical allodynia induced by streptozocin in the rat. Pain, 1999, 80(1-2), 391-398.
[http://dx.doi.org/10.1016/S0304-3959(98)00239-5] [PMID: 10204753]
[31]
Taylor, J.L.; Amann, M.; Duchateau, J.; Meeusen, R.; Rice, C.L. Neural contributions to muscle fatigue: From the brain to the muscle and back again. Med. Sci. Sports Exerc., 2016, 48(11), 2294-2306.
[http://dx.doi.org/10.1249/MSS.0000000000000923] [PMID: 27003703]
[32]
Kim, Y.H.; Back, S.K.; Davies, A.J.; Jeong, H.; Jo, H.J.; Chung, G.; Na, H.S.; Bae, Y.C.; Kim, S.J.; Kim, J.S.; Jung, S.J.; Oh, S.B. TRPV1 in GABAergic interneurons mediates neuropathic mechanical allodynia and disinhibition of the nociceptive circuitry in the spinal cord. Neuron, 2012, 74(4), 640-647.
[http://dx.doi.org/10.1016/j.neuron.2012.02.039] [PMID: 22632722]
[33]
Bradesi, S. Role of spinal cord glia in the central processing of peripheral pain perception. Neurogastroenterol. Motil., 2010, 22(5), 499-511.
[http://dx.doi.org/10.1111/j.1365-2982.2010.01491.x] [PMID: 20236247]
[34]
Zhou, Y.Q.; Liu, Z.; Liu, Z.H.; Chen, S.P.; Li, M.; Shahveranov, A.; Ye, D.W.; Tian, Y.K. Interleukin-6: An emerging regulator of patho-logical pain. J. Neuroinflammation, 2016, 13(1), 141.
[35]
Halagappa, K.; Girish, H.N.; Srinivasan, B.P. The study of aqueous extract of Pterocarpus marsupium Roxb. on cytokine TNF-α in type 2 diabetic rats. Indian J. Pharmacol., 2010, 42(6), 392-396.
[http://dx.doi.org/10.4103/0253-7613.71922] [PMID: 21189913]
[36]
Chakraborty, A.; Gupta, N.; Ghosh, K.; Roy, P. In vitro evaluation of the cytotoxic, anti-proliferative and anti-oxidant properties of pterostilbene isolated from Pterocarpus marsupium. Toxicol. In Vitro, 2010, 24(4), 1215-1228.
[http://dx.doi.org/10.1016/j.tiv.2010.02.007] [PMID: 20152895]
[37]
Deshmukh, P.; Unni, S.; Krishnappa, G.; Padmanabhan, B. The Keap1-Nrf2 pathway: Promising therapeutic target to counteract ROS-mediated damage in cancers and neurodegenerative diseases. Biophys. Rev., 2017, 9(1), 41-56.
[http://dx.doi.org/10.1007/s12551-016-0244-4] [PMID: 28510041]
[38]
Chakraborty, P.; Saraswat, G.; Kabir, S.N. α-Dihydroxychalcone-glycoside (α-DHC) isolated from the heartwood of Pterocarpus marsupium inhibits LPS induced MAPK activation and up regulates HO-1 expression in murine RAW 264.7 macrophage. Toxicol. Appl. Pharmacol., 2014, 277(1), 95-107.
[http://dx.doi.org/10.1016/j.taap.2014.03.011] [PMID: 24675710]
[39]
Mohammadi, M.; Khole, S.; Devasagayam, T.P.; Ghaskadbi, S.S. Pterocarpus marsupium extract reveals strong in vitro antioxidant activity. Drug Discov. Ther., 2009, 3(4), 151-161.
[PMID: 22495601]
[40]
Hougee, S.; Faber, J.; Sanders, A.; de Jong, R.B.; van den Berg, W.B.; Garssen, J.; Hoijer, M.A.; Smit, H.F. Selective COX-2 inhibition by a Pterocarpus marsupium extract characterized by pterostilbene, and its activity in healthy human volunteers. Planta Med., 2005, 71(5), 387-392.
[http://dx.doi.org/10.1055/s-2005-864130] [PMID: 15931573]
[41]
Lopes, S.C.; da Silva, A.V.L.; Arruda, B.R.; Morais, T.C.; Rios, J.B.; Trevisan, M.T.S.; Rao, V.S.; Santos, F.A. Peripheral antinociceptive action of mangiferin in mouse models of experimental pain: Role of endogenous opioids, K(ATP)-channels and adenosine. Pharmacol. Biochem. Behav., 2013, 110, 19-26.
[http://dx.doi.org/10.1016/j.pbb.2013.05.016] [PMID: 23747933]
[42]
Florentino, I.F.; Nascimento, M.V.; Galdino, P.M.; De Brito, A.F.; Da Rocha, F.F.; Tonussi, C.R.; De Lima, T.C.M.; De Paula, J.R.; Costa, E.A. Evaluation of analgesic and anti-inflammatory activities of Hydrocotyle umbellata L., Araliaceae (acariçoba) in mice. An. Acad. Bras. Cienc., 2013, 85(3), 987-997.
[http://dx.doi.org/10.1590/S0001-37652013000300011] [PMID: 24068088]
[43]
Menorca, R.M.G.; Fussell, T.S.; Elfar, J.C. Nerve physiology: Mechanisms of injury and recovery. Hand Clin., 2013, 29(3), 317-330.
[http://dx.doi.org/10.1016/j.hcl.2013.04.002] [PMID: 23895713]
[44]
Varejão, A.S.P.; Cabrita, A.M.; Meek, M.F.; Bulas-Cruz, J.; Melo-Pinto, P.; Raimondo, S.; Geuna, S.; Giacobini-Robecchi, M.G. Functional and morphological assessment of a standardized rat sciatic nerve crush injury with a non-serrated clamp. J. Neurotrauma, 2004, 21(11), 1652-1670.
[http://dx.doi.org/10.1089/neu.2004.21.1652] [PMID: 15684656]
[45]
Doolen, S.; Blake, C.B.; Smith, B.N.; Taylor, B.K. Peripheral nerve injury increases glutamate-evoked calcium mobilization in adult spinal cord neurons. Mol. Pain, 2012, 8(1), 56.
[http://dx.doi.org/10.1186/1744-8069-8-56]
[46]
Görlach, A.; Bertram, K.; Hudecova, S.; Krizanova, O. Calcium and ROS: A mutual interplay. Redox Biol., 2015, 6(1), 260-271.
[http://dx.doi.org/10.1016/j.redox.2015.08.010] [PMID: 26296072]
[47]
Verkhratsky, A.; Fernyhough, P. Calcium signalling in sensory neurones and peripheral glia in the context of diabetic neuropathies. Cell Calcium, 2014, 56(5), 362-371.
[http://dx.doi.org/10.1016/j.ceca.2014.07.005] [PMID: 25149565]

Rights & Permissions Print Cite
Article Metrics
13
Wayfinder Image
Open Access Journals Promotions
© 2024 Bentham Science Publishers | Privacy Policy