Title:Oral Biofilm on Dental Materials Among Khat Chewers
Volume: 21
Issue: 10
Author(s): Mohammed M. Al Moaleem*, Amit Porwal, Nasser M. Al Ahmari and Mansoor Shariff
Affiliation:
- Prosthetic Dental Science Department, College of Dentistry, Jazan University, Jazan 45142,Saudi Arabia
Keywords:
Khat, prosthetic materials, restorative materials, streptococcus, veillonella, oral biofilm.
Abstract:
Background: Nowadays khat chewing habit is increasing among population in southern part
of Saudi Arabia, Jazan and till date there is no literature investigating the effect of khat on oral biofilm
on dental materials.
Objective: To evaluate and compare the bacterial biofilm on different types of dental restorative materials
used in replacing missing tooth structures among khat chewers and non-khat chewers.
Materials and Methods: Hundred and twenty biofilm samples were collected from different dental restorations,
such as All-ceramic (AL), Metal Ceramic (MC), Metal crowns or bridges (M), Composite
(C), Glass Ionomer (GI) and Amalgam (A) restorations in non-khat and khat chewers (K). DNA extraction
was done and subjected to PCR. Bacterial species, such as Streptococcus, Neisseria, Bacillus,
Granulicatella and Veillonella were identified and counted. PCR products were also sequenced to detect
similarity. Association between bacterial type and dental materials among non-khat and khat
chewers were tested with Chi-Square test (Fishers Exact test).
Results: The frequency and percentage of Streptococcus species were marginally higher among khat
chewers (42; 70%) compared with non-khat chewers (38; 63.3%) group. But the Veillonella species
were higher among non-khat chewers (9: 15%), compared to the khat chewers group (7; 11.7%). No
statistically significant difference was detected among species in both groups. In non-khat and khat
chewer group, the maximum hits were related to Streptococcus spp. in glass ionomer, amalgam, and
composite (restorative materials), followed by metal ceramic and metal (prosthetic materials). Veillonella
spp. showed maximum hits in the metal group among non-khat chewers and in all-ceramic
among khat chewers. Statically significant differences were recorded among composite and amalgam
samples with p values 0.047 and 0.036 in khat chewer group.
Conclusion: Khat chewers showed statistically significant differences in oral biofilm in the composite
and amalgam restorative materials, but there were no significant differences found among any materials
and species between the groups.
