Methods to recover genetic material with the best possible quality have been
improving in a similar way to the other areas in this field, culminating with the
obtention of the first complete ancient mitogenome in 2001. However, with the arrival
of next-generation sequencing in 2005, all these advances can be considered overcome.
Using shotgun sequencing as basis and specially-designed microbeads to attach DNA,
the advent of the palaeogenomics era has revolutionized the field. Also the enrichment
techniques and the growing knowledge of ancient DNA diagenesis add significant
achievements, but then again the roof of this technology has yet to be attained.
Keywords: Amelocementary limit, Bleach, DNA extraction, Enrichment, Library
preparation, Medullary canal, Microbeads, Mitogenomes, Next-generation
sequencing, Pleistocene, Pre-treatment, Priming sites, Purification.
