Transplantation of mesenchymal stem/stromal cells (MSCs) is an emerging
treatment for various hard-to-treat diseases. In recent decades, cell sheet technology
(CST) has been exploited as a novel promising scaffold-free approach for cell culturing
and delivering in tissue regeneration. Results have demonstrated that MSCs facilitate
cell viability and function in the CST co-culture system to improve transplantation
efficiency. There are extensive interests in developing strategies to enhance the
formation of cell sheets with MSC for downstream applications. Novel fabrication
techniques have been emerging in recent years, including temperature-responsive
system, electroactive responsive system, oxide surface assistant electrochemical
system, light responsive system, PH responsive system, magnetite nanoparticles and
magnetic force system. This chapter summarizes recent studies in cell sheet
engineering and its combination with MSC for tissue regeneration.
Keywords: Cell sheet technology, Co-culture, Mesenchymal stem cell, Micropatterning,
Tissue engineering, Vascularization.
