Cellulases comprise an important group of enzymes involved in several
industrial processes. In recent years they are especially being used in the lignocellulosic
biomass conversion into small sugars to produce cellulosic ethanol and chemicals.
Filamentous fungi are the most important producers of these catalysts. In order to
increase cellulase yield and reduce the production costs, many strategies are being
applied such as, submerged fermentation or solid state fermentation using waste
residues, aligned with advanced molecular techniques to improve the enzymes
performance, reducing its amounts in industrial process as well as the time required by
its production. Thus, this chapter will present some of the most important procedures to
scale up cellulase production to enable second generation industry.
Keywords: Cellulase production, enzymatic hydrolysis, lignocellulosic residues,
scale up.