The aim of this chapter is to present the modern analytical methodologies used to study peptidases, such as the colorimetric methods and spectrophotometric analyses. A general method for the detection of peptidases activity includes gelatin and other substrates incorporated into SDS-PAGE using a technique known as zymography. All methodologies presented here are important tools for the discovery and characterization of peptidases.
Keywords: Peptidase, protease, gelatin, metallopeptidases, isopropanol, zymography.
