Ocular samples include: ocular cells or tissue (conjunctiva, cornea, retina, or orbital tissue), bio-fluids
such as tears, aqueous or vitreous, therapeutic or cosmetic contact lenses, implanted devices (intraocular lenses,
corneal rings), or transplanted tissue (e.g. Infected corneal graft tissue). Sampling extends beyond the careful
collection of ocular material to involve sample storage and transfer. Reliable results require avoiding sample
contamination at any stage of sampling, storage, and processing. In order to aid the laboratory scientist to provide
an accurate interpretation of the investigations, it is extremely important to give details of all relevant clinical
history, other related medical conditions, clinical differential diagnosis, and in some cases demographical details
such as race, birth place and current postcode. This chapter will provide a précis of common sample collection in
ophthalmology and an overview of culture-dependent and independent techniques including immunohistochemistry
and molecular biological techniques used for ophthalmological diagnosis.
Keywords: Laboratory diagnosis, sampling, tears, aqueous humour, vitreous humour, sampling, culture,
immunohistochemistry, molecular biology, gram stain, cytology, microscopy, histopathology, histology,
microbiology, virology, biopsy.
