CRISPR-Cas technology has reshaped the field of microbiology. It has
improved the microbial strains for better industrial and therapeutic utilization. In this
chapter, we have tried to provide an overview of this technology with special reference
to its associated applications in the various fields of interest. We have discussed the
origin, classification, and different genome editing methods of CRISPR-Cas to
understand its historical significance and the basic mechanism of action. Further,
different applications in the area of agriculture, food industry, biotherapeutics, biofuel,
and other valuable product synthesis were also explained to highlight the advancement
of this system in industrial microbes. We have also tried to review some of the
limitations offered by CRISPR and insights into its future perspective.
Keywords: Agriculture, Biofuel, Biotherapeutics, CRISPR/Cas9, Food industry, Genome editing, Industrial applications, Microorganisms.
