This chapter gives examples of the use of standard and advanced light microscopy techniques to
visualize the interaction of selected pathogens with neutrophils in living animals. To date the knowledge on the
dynamic interaction of invading pathogens with immune cells is at the very beginning. We focus on recent studies
of the parasites Toxoplasma, Plasmodium and Leishmania in laboratory mice and the bacterial agent
Pseudomonas in zebrafish. In addition we discuss the basic imaging requirements and some technical pitfalls that
can occur during image acquisition and data analysis. Advanced imaging approaches have become an essential
tool in modern cell biology, which is also reflected by the rapid development of new high-tech equipment.
