In-flow methodologies have allowed the development of less timeconsuming
analytical techniques requiring small volumes of real samples based on
microfluidic channels, microfluidic and nanofluidic devices coupled to different optical
setups. Moreover, these in-flow systems have led to the confinement of varied
nanostructures and functions. Thus, from multifunctional nanoparticles to labelled
biostructures, depending on the coupled detection systems, varied signals could be
recorded. Likewise, chemical reactions and surface modifications could be developed
looking for targeted functional modifications within in-flow methodologies.
Accordingly, the detection of single molecules on lab-on particles to single targeted
nanostructures, microparticles, bacteria and cells could be recorded from new modes of
imaging generated from the control of molecules, surfaces and nanostructures within in
flow nano- and micro-channels. Chemical surface modifications could also lead to
additional physical sites of interactions and property coupling for real time biosensing.
Keywords: Coupled optical setups, Cytometry, In-flow methodologies,
Microfluidic chips, Microfluidics, Nanofluidics.
