An enzyme biosensor is an investigative device that joins an enzyme with a
transducer to create a signal corresponding to target analyte fixation. An ideal enzyme
activity is a basis for the maintenance of physiological homeostasis of biosensor. Both
non-hereditary and hereditary disturbances can too much initiate or quiet characteristic
enzyme activities. Due to its virtuous sensitivity, easy to operate, high precision and
low instrumentation cost it can be used for recognition of various analytes in different
fields than the traditional analytical methods. The enzyme based biosensor is most
commonly used dominant tool for the determination of various biological importance
such as antigens, antibodies, therapeutic drugs and metabolites. In regular enzymebased
biosensors, signal amplification is not satisfactory for the ultrasensitive
identification of biomolecules and it was enhanced by consolidating enzymatic
responses with redox cycling of multi-enzyme tags for every discovery test. This
chapter explains the working principles, features, types, sensing methods and
applications of enzyme based biosensor in various fields.
Keywords: Antigen, Antibody, Biomolecules, Chemical activators,
Electrochemical, Enzyme biosensor, Homeostasis, Hereditary, Inhibitor,
Metabolite, Nanomaterials, Transducer, Therapeutic drugs.
