Soybean (Glycine max) is one of the legumes, susceptible to yellow mosaic
disease caused by Mungbean yellow mosaic India virus (MYMIV) and Mungbean
yellow mosaic virus (MYMV) infection. The quantitative proteomic analysis allows
achieving deeper knowledge about the viral infection. For quantitative proteomic
analysis, two-dimensional gel electrophoresis (2D-PAGE) is the common method of
choice. Optimization is required even for the published protocols based on the type of
sample to be analyzed and for the proteins of interest. We compared four different
published protocols with some modifications and selected the one which is more
effective in terms of resolution and reproducibility of 2D-PAGE. Here we present our
simple and cost-effective procedure for the detection of viral infection and proteomic
analysis of YMV infected soybean leaves without compromising the resolution and
reproducibility of 2D-PAGE.
Keywords: Glycine max, Leaf proteome, Mungbean yellow mosaic India virus,
Protein extraction, Soybean, Two-dimensional gel electrophoresis, Yellow mosaic
disease.
