Field-Flow Fractionation techniques (FFF) are size-based separation
methods first described in 1966 by Giddings. They belong to the family of liquid
chromatographic techniques, but they are operated without any stationary phase.Yet,
they have the unique ability to separate an extremely broad range of molecules,
macromolecules and particles, and in particular very large particles, with a high
resolution. FFF are versatile: by varying the experimental conditions, the range, speed,
and power of the separation could be optimized. FFF techniques can succeed when
Size-Exclusion Chromatography (SEC) methods fails, and they have a broad range of
applications. In this chapter the theory of FFF is approached together with calibration
and determination of some structural parameters such as size and molar mass, the
instrumentation and detection of various classic FFF types is described and we show
the added value of FFF techniques for the characterization of various biopolymers
including polysaccharides, proteins and natural rubber.
Keywords: Aggregation, Field-Flow Fractionation, Multiangle Laser Light
Scattering, Natural Rubber, Polysaccharides, Proteins, Starch, Static and Dynamic
Light Scattering
