Small-angle scattering of X-rays (SAXS) or neutrons (SANS) is a wellestablished
method to study the overall structure and structural transitions of
biopolymers in solution. Determination of overall structural parameters such as the
radius of gyration Rg, the molecular mass Mw or the hydrated volume V is now easily
computed using appropriate free Softwares. In addition, increasingly sophisticated ab
initio methods were developed over the last two decades for building structural models
of biopolymers from x-ray and neutron solution scattering data. Even if most of the
developments were focused on protein in solution, folded or intrinsically disordered,
protein complexes or protein fibrils, ab initio methods were also used to calculate
three-dimensional shape models of polysaccharides or nucleic acids. The models
derived from experimental scattering pattern up to a resolution of 0.5 nm are classified
as low resolution models compared to atomistic resolution structures using X-ray
crystallography. The efficiency of the methods is illustrated in this chapter by focusing
mainly on proteins or polysaccharides with unknown crystal structures and for which
the ab initio reconstruction of three-dimensional models may help to define, in
combination or not with other structural techniques, such as microscopy, overall
dimensions and global shape. These methods improved the resolution and reliability of
models derived from scattering data substantially and has made solution scattering, in
combination with recent developments using size-exclusion chromatography, robotic
sample changer or microfluidics, and a useful technique in high-throughput large-scale
structural characterization of biopolymers.
Keywords: Ab initio, Complexes, Fibrils, Flexibility, Microfluidics, Modeling,
Neutron, Pair distance distribution function, Polydispersity, Polysaccharide,
Proteins, Radius of gyration, Sample changer, Size exclusion chromatography,
Small-angle scattering, Three-dimensional structures, X-ray.
