Title:Association of CYP2C19 and HSP70 Genes Polymorphism with Aspirin- Exacerbated Respiratory Disease in a Kurd Population
Volume: 20
Issue: 2
Author(s): Wesam Kooti, Mohammad Abdi, Yashpal S. Malik, Bijan Nouri, Ali Jalili, Mohammad A. Rezaee, Mohammad R. Rahmani*Rasoul N. Kalmarzi*
Affiliation:
- Lung Diseases and Allergy Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj,Iran
- Lung Diseases and Allergy Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj,Iran
Keywords:
Polymorphism, PCR-RFLP, HRM, aspirin-exacerbated respiratory disease, heat shock proteins, asthma,
kurdistan.
Abstract:
Background: CYP2C19 a metabolizing enzyme and Heat Shock Proteins (HSP) are induced
in stress conditions, such as hypoxia and ischemia. Recently, polymorphism in the CYP2C19 and HSP
genes has been established in Aspirin-Exacerbated Respiratory Disease (AERD).
Objective: We investigated the polymorphism of these two genes in Kurdish patients with AERD.
Methods: This study involved 306 subjects, referred to the Be’sat hospital in Kurdistan Province,
which were divided into three groups, (i) Aspirin Induced Asthma (AIA), (ii) Aspirin Tolerant Asthma
(ATA), and (iii) healthy subjects as control. The subjects as control and ATA\AIA groups were verified
by the physician. The demographic data of each subject with respect to age, sex, parental
education, and residence was collected. Spirometry was performed on subjects and blood samples were
collected for serum Immunoglobulin E (IgE) estimation and molecular tests. Genotyping was done for
CYP2C19 681G>A، CYP2C19 636G>A, and HSPA1B1267A>G by using PCR- Restriction Fragment
Length Polymorphism (RFLP) and for HSPA1B-179C>T by High Resolution Melting (HRM).
Results: Demographic statistics were not significantly different between the three groups (p>0.05).
Further, genotypes were also not observed to be significantly different in the genes of CYP2C19
681G>A, CYP2C19 636G>A and HSPA1B1267A>G (p>0.05). However, the heterozygote genotype
in HSPA1B-179 C>T in AIA group was higher than the control group (p<0.05). Notably, 92.8 % of the
subjects showed heterozygote genotype in HSPA1B1267 A>G. In clinical tests, FEV-1, FVC, and
asthma severity in the AIA group were higher than control and additionally IgE levels were lower in
this group (p<0.05).
Conclusion: The results confirm the association of polymorphism in the HSPA1B-179C>T and
HSPA1B1267A>G with AERD in the Kurdish population.
